Sequences from confirmation amplicon


Confirmation sequences for line 367G05 and hit At1g19150 are shown.
Line ID 367G05
Locus At1g19150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6613795
Primers used for PCR DE32 - 8474, show primer details
   
Sequence Name 47-K027346-022-367-G05C-8409
GenBank Accession LN506079 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K027346-022-367-G05C-8409
ATTGAATATATCCTGCAATATGTTTGTTGTGAATGTAAATGCCTGCAAAAGAAGTACGTA
CCAATGAAGTCTTATCAATACACATAACACATATCATCACCATGAATCTATGATAAATGA
TCATAACAATTTTAACTCACCGAAAAGACGTTGCAATGACCAGGATCAGCCAGATGAGCC
ATGAGATTCTCAATTGGATCTTGGCTAGTGTAGGTAGCTTGGAAACAGAACCCAAGGAAA
GCAAGCATCGCGAGCCGTCCGTTCTTAATCTCTTTAGTCCTCAAAACCATTACCGGTTCA
GGAGAACCTCTCCCCCACATCATAAAATCGAACCACAAACCTCCAGGGTAACCAACATCC
GGTTTAGGATTTACTTTATGCGGGTACTTTGGTTCTATGTCAACAGACCCCGGTTTAATC
AAATCAGCCCATCTTCTACCTTCTGCCCAGCCCATTAAAACCAT
   
Sequence Name 48-K027346-022-367-G05C-DE32
GenBank Accession LN506396 [GenBank]
Sequencing primer gene specific primer, DE32: 5'-GTTTGTGGCTCAAATGGTTTTAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K027346-022-367-G05C-DE32
GGGCTGATTTGATTAAACCGGGGTCTGTTGACATAGAACCAAAGTACCCGCATAAAGTAA
ATCCTAAACCGGATGTTGGTTACCCTGGAGGTTTGTGGTTCGATTTTATGATGTGGGGGA
GAGGTTCTCCTGAACCGGTAATGGTTTTGAGGACTAAAGAGATTAAGAACGGACGGCTCG
CGATGCTTGCTTTCCTTGGGTTCTGTTTCCAAGCTACCTACACTAGCCAAGATCCAATTG
AGAATCTCATGGCTCATCTGGCTGATCCTGGTCATTGCAACGTCTTTTCGGTGAGTTAAA
ATTGTTATGATCATTTATCATAGATTCATGGTGATGATATGTGTTATGTGTATTGATAAG
ACTTCATTGGTACGTACTTCTTTTGCAGGCATTTACATTCACAACAAACATATTGCAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATT

Last Updated on 06.12.2018 16:11