Sequences from confirmation amplicon


Confirmation sequences for line 367H02 and hit At2g39740 are shown.
Line ID 367H02
Locus At2g39740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16576720
Primers used for PCR 11du - 8474, show primer details
   
Sequence Name 75-K110363-0022-367-H02C-11du
GenBank Accession LN513259 [GenBank]
Sequencing primer gene specific primer, 11du: 5'-ATATATCACTCTTAGAGGCGGCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>75-K110363-0022-367-H02C-11du
ACAACACATAGAACATCTTACTATGGTTTCTAATGATGCAGTAACTGCAGAAGGCTGAAG
GCAGCAAAATCTATGAATACAGAACAGCTATCTTACCTGAAAATGGAAGATGACTAACAA
ACTGAGAGAGTAAGAGTTGAAAGTCCCGGTCTTTGAGTCGTTGATATTATGAGCTTTCGC
CCATTCCTTCACCTACAAATGTAGAATACACGAGGTAGATACGTTTAGAATCAATTAAAC
CTTTGGAAACACACAATATGGTTTTATTTGCATGTTAACTTACTAGCAAAACCAGGTCGC
GGAATCTCCCATCTATTTCACTGATCCAGAACAAGAACCTGCTCTTCAAAAGCCCATCCA
GGTTATCAATAGAAATGTCACAAGAAATTCTCTGGTGGCCGCTCACTACTTTCAGAATAG
GAACTCGCGCATGAATAACAAATTGCAGTTTGTACCATAGACCTGTTTTTTGAATGATAA
TGGATGTGAGACAAATCGGCACATGAAATAATACTTAAACTACTTAAGTAGTATATAAGG
TAAACAACAGTGAAGGCAAGAGAAAACCCAAAAAAGAAGAAAAAAATAGAAGCTTAATGA
ATCACCACTCGCTCTCAGTGCTCGTAGTAAATGTCCGAGTAAAGTTTGGTTCTGCTTCCT
TC
   
Sequence Name 67-K110362-0022-367-H02C-8409
GenBank Accession LN511837 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K110362-0022-367-H02C-8409
ATTTACAATTGAATCTCTGTTGACTTATTCAGCGGTTCTTCCATCTTGTTCACTGGAAAG
AAGCAGAAACAAACTTTACTCGGACATTTACTACGAGCACTGAGAGCGAGTGGTGATTCA
TTTAGCTTCTATTTTTTTCTTCTTTTTTTGGTTTTCTCTTTGCTTCACTGTTGTTTACCT
TATATACTACTTAAGTAGTTTAAGTATTATTTCATGTGCCGATTTGTCTCACATCCATTA
TCATTCAAAAAACAGGTCTATGGTACAAACTGCAATTTGTTATTCATGCGCGAGTTCCTA
TTCTGAAAGTAGTGAGCGGCCACCAGAGAATTTCTTGTGACATTTCTATTGATAACCTGG
ATGGGCTTTTGAAGAGCAGGTTCTTGTTCTGGATCAGTGAAATAGATGGGAGATTCCGCG
ACCTGGTTTTGCTAGTAAGTTAACATGCAAATAAAACCATATTGTGTGTTTCCAAAGGTT
TAATTGATTCTAAACGTATCTACCTCGTGTATTCTACATTTGTAGGTGAAGGAATGGGCG
AAAGCTCATAATATCAACGACTCAAAGACCGGGACTTTCAACTCTTACTCTCTCAGTTTG
TTAGTCATCTTCCATTT

Last Updated on 06.12.2018 16:11