Sequences from confirmation amplicon


Confirmation sequences for line 368F01 and hit At1g04920 are shown.
Line ID 368F01
Locus At1g04920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1392372
Primers used for PCR TH12 - 8474, show primer details
   
Sequence Name 80-K103584-0022-368-F01C-TH12
GenBank Accession LN513941 [GenBank]
Sequencing primer gene specific primer, TH12: 5'-GACTTACCTTACACGTATAGCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K103584-0022-368-F01C-TH12
ACGATGAATTTTCACTCACGACTCTCTTAAAGACGTTCAAGACATGTCTCTGAGACTCTC
TATGGATGGAGATAAACCATCGTTGAACGGGTCTCTTGAACCGAATTCTGCTGATCCGGT
TAAACAAATCATGAGCCGGATGAGGACACCTGAAATCAAGAGTAAACCAGAATTACAGGG
GAAGAAACAAAGTGATAACTTGGGTAGCAAGTATCCGGTTTTGAGGCGACGGGAACGGTT
GGTGGTTTTAGCGGTTGATTGCTATGACAATGAGGGAGCTCCGGATGAGAAAGCAATGGT
TCCGATGATTCAGAATATCATCAAAGCCGTGCGATCGGATCCACAGATGGCTAAAAACTC
TGGTTTTGCGATTTCCACTTCTATGCCGCTTGATGAATTAACTCGCTTCTTGAAGTCTGC
CAAGATTCAGGTGAGTGAGTTCGACACATTAATATGCAGCAGCGGGAGCGAAGTGTATTA
TCCAGGAGGGGAAGAGGGAAAGCTTCTTCCGGATCCTGACTATTCCTCGCATATCGATTA
CCGATGGGGCATGGAAGGGCTTAAGAACACGGTGTGGAAACTGATGAAAAAGAAAGCAAT
AAACAAATGATCATCAATTGAATATGCGATTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGT
   
Sequence Name 86-K103585-0022-368-F01C-8409
GenBank Accession LN514649 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K103585-0022-368-F01C-8409
ATATTCAATTGATGATCATTTGTTTATTGCTTTCTTTTTCATCAGTTTCCACACCGTGGT
TCTTAAGCCCTTCCATGCCCCATCGGTAATCGATATGCGAGGAATAGTCAGGATCCGGAA
GAAGCTTTCCCTCTTCCCCTCCTGGATAATACACTTCGCTCCCGCTGCTGCATATTAATG
TGTCGAACTCACTCACCTGAATCTTGGCAGACTTCAAGAAGCGAGTTAATTCATCAAGCG
GCATAGAAGTGGAAATCGCAAAACCAGAGTTTTTAGCCATCTGTGGATCCGATCGCACGG
CTTTGATGATATTCTGAATCATCGGAACCATTGCTTTCTCATCCGGAGCTCCCTCATTGT
CATAGCAATCAACCGCTAAAACCACCAACCGTTCCCGTCGCCTCAAAACCGGATACTTGC
TACCCAAGTTATCACTTTGTTTCTTCCCCTGTAATTCTGGTTTACTCTTGATTTCAGGTG
TCCTCATCCGGCTCATGATTTGTTTAACCGGATCAGCAGAATTCGGTTCAAGAGACCCGT
TCAACGATGGTTTATCTCCATCCATAGAGAGTCTCAGAGACATGTCTTGAACGTCTTTAA
GAGAGTCGTTGAGTGAAAATTCATCGTCTTGAGCAGCCACTTCGTCCGCATCTGTCTGCC
ACTGCGGATGTCTCATACGGCACGCGGCTATAC

Last Updated on 06.12.2018 16:11