Sequences from confirmation amplicon


Confirmation sequences for line 368G06 and hit At5g39430 are shown.
Line ID 368G06
Locus At5g39430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15776391
Primers used for PCR 23aq - 8474, show primer details
   
Sequence Name 07-K106755-0022-368-G06C-8409
GenBank Accession LN489289 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K106755-0022-368-G06C-8409
TTACATTGAATAATTCATTTACATGAAGCCATTTATAGATAAACGAAAACACCCGGCTCC
AAACCAATGTCCATACACTCCAATTGGTGTTGACTTGTTTGTGTGTCCAAGGAAGATTGA
TCATATAGCCCAACACATTGAGCTTCCCAACATCAAAGCTGTGGCAAACCTCCCTGCTCT
TCTAATTGTCAACATCCAGGTAAAAACCACTAAGAAAGTTTCTTTAGATCAAAAGAGATG
TGTGGTAAAGACTCTAATGAATTTGGCTTTTGCAGTTGCCAACTTATCCAGCCGCAATGT
TTCTCGGTGATAGTAACGGAGAAGGCATGAGCATTGTACTTTACTTTAAACTACGAGAAA
ATTTCAAAAATGAAATCTCTCAACAATACCAGAACAGCA
   
Sequence Name 23-K106756-0022-368-G06C-23aq
GenBank Accession LN496573 [GenBank]
Sequencing primer gene specific primer, 23aq: 5'-GATGCTGTCTTGGTATTGTTGAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K106756-0022-368-G06C-23aq
GAAGTACAATGCTCATGCCTTCTCCGTTACTATCACCGAGAAACATTGCGGCTGGATAAG
TTGGCAACTGCAAAAGCCAAATTCATTAGAGTCTTTACCACACATCTCTTTTGATCTAAA
GAAACTTTCTTAGTGGTTTTTACCTGGATGTTGACAATTAGAAGAGCAGGGAGGTTTGCC
ACAGCTTTGATGTTGGGAAGCTCAATGTGTTGGGCTATATGATCAATCTTCCTTGGACAC
ACAAACAAGTCAACACCAATTGGAGTGTATGGACATTGGTTTGGAGCCGGGTGTTTTCGT
TTATCTATAAATGGCTTCATGTAAATGAATATATTCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
Primers used for PCR 01ax - 3144, show primer details
   
Sequence Name 05-K106867-0022-368-G06C-3144
GenBank Accession LN488374 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K106867-0022-368-G06C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTAAAAAATTGGTAATTACTCTTTCTTTT
TCTCCATATTGACCATCATACTCATTGCTGATCCATGTAGATTTCCCGGACATGAAGCCA
TTTACAATTGAATATATCCTGTCCAATTGTATACAAATTTATCATTGTATTAACAGTCTA
CATCCACTTAGAGCCGGTTAAATGCATACATATATGTATATATAAACATAGAAGTTAAGG
AATATACTTGAAATAAGTTTCGCCACGTAACTTCAAGTTTGATGGAGGTATCTCACACCA
ACTTCCTGATGATTGATGCTTCTCTTTGACATAGCACGGAATCGTGTATCCGGCTTTGGG
ACGGTACAACAATCTTTTCGACGACCCTTTATATAAAAACCATTTCAAAATATACATGTT
AAAATTACAGGCTCACATAAGAATGCATATATACATGTTTTGATGTTGTACGGTTTAGTA
AAAAATTTGACAACTTACTATGTTCGGTAACTTCTTCACACAT
   
Sequence Name 15-K106868-0022-368-G06C-01ax
GenBank Accession LN492992 [GenBank]
Sequencing primer gene specific primer, 01ax: 5'-GAGAAGATCATGTGATGGTGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K106868-0022-368-G06C-01ax
GTTGTCAATTTTTACTAACCGTACACATCAAAACATGTATATATGCATTCTTATGTGAGC
CTGTAATTTTAACATGTATATTTTGAAATGGTTTTTATATAAAGGGTCGTCGAAAAGATT
GTTGTACCGTCCCAAAGCCGGATACACGATTCCGTGCTATGTCAAAGAGAAGCATCAATC
ATCAGGAAGTTGGTGTGAGATACCTCCATCAAACTTGAAGTTACGTGGCGAAACTTATTT
CAAGTATATTCCTTAACTTCTATGTTTATATATACATATATGTATGCATTTAACCGGCTC
TAAGTGGATGTAGACTGTTAATACAATGATAAATTTGTATACAATTGGACAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTAACCGAAGGCGGGAAACGACAAT
CTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGG
TCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATAT

Last Updated on 06.12.2018 16:11