Sequences from confirmation amplicon


Confirmation sequences for line 369A05 and hit At4g31760 are shown.
Line ID 369A05
Locus At4g31760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15368677
Primers used for PCR GE05 - 8474, show primer details
   
Sequence Name 19-K033705-022-369-A05C-8409
GenBank Accession LN494662 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K033705-022-369-A05C-8409
TGATGTAAATAAAAAAAGAAGAAGCTGAGTGTAACAGAATATGAAACAGATTTTGCTACA
AGAATTGACTTTACCATAGTTAAAGAAAAAAAACTCTGTTTCATACTCTGTTTGCGTTTA
GCAAAACATGTTGGTAAAATTGATTAGAAGTTTCAAGAAACGTTGATATATACCTGAGAG
GACGACGAGATCTTGAATGGACAAGCCTTTGGAAGAGAAGGCATCGATCATTTGATCGAG
AGTAAAATCTGTGTCGATGATGTTCGGTCTCACGTTTGCAGCCATCGATTCTTTCCCGTC
TCTCCTCCCTGTCGGAATCTCAACCACCGGTCCTCCTGCCTGTCCCGCCAAAGAAGAAAA
ACATTCAAACCTTAATAAGGATAACCGGTTTAATGTATCTACAACTCCGGTTATATTTGG
TTCATTGTGTAGTCTATGCGAAAATGATACAGAATTGGTTTGTTTCTTTTTTTCGATTTC
ATGTGTATGCTTTCGGTTCAGTTGGGATTGGTTAAACCGGATTAGAGCAATATAGATTGA
AGTGAAGTGGGAGTGAAGTAAATGTTACAGCTTCGACGGCGTCTCTAGCAGCAAGTGCAA
CTATGTCAGCGCAAGAAACAGTGGCCGGACATAAATTCTCGATGGCATTCTTGGCTGTGT
CTATGACTGAGAATCCTCCAAGAGATGCGTTTCCGGGATCGCTTTTCTCTGTGCTATTCC
CTTGTATCAACACTGATGCATCACAACCCTTGCAAGTTCAACCAACAAACAAAGTTTAAG
CCAAATTGTTTGAACTTTCCTCAGTAATTCGTAATTAGTGC
   
Sequence Name 19-K033705-022-369-A05C-GE05
GenBank Accession LN494663 [GenBank]
Sequencing primer gene specific primer, GE05: 5'-CAGGTAACACGCACTAATTACGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K033705-022-369-A05C-GE05
CATTTGGCTTAACTTTGTTTGTTGGTTGAACTTGCAAGGGTTGTGATGCATCAGTGTTGA
TACAAGGGAATAGCACAGAGAAAAGCGATCCCGGAAACGCATCTCTTGGAGGATTCTCAG
TCATAGACACAGCCAAGAATGCCATCGAGAATTTATGTCCGGCCACTGTTTCTTGCGCTG
ACATAGTTGCACTTGCTGCTAGAGACGCCGTCGAAGCTGTAACATTTACTTCACTCCCAC
TTCACTTCAATCTATATTGCTCTAATCCGGTTTAACCAATCCCAACTGAACCGAAAGCAT
ACACATGAAATCGAAAAAAAGAAACAAACCAATTCTGTATCATTTTCGCATAGACTACAC
AATGAACCAAATATAACCGGAGTTGTAGATACATTAAACCGGTTATCCTTATTAAGGTTT
GAATGTTTTTCTTCTTTGGCGGGACAGGCAGGAGGACCGGTGGTTGAGATTCCGACAGGG
AGGAGAGACGGGAAAGAATCGATGGCTGCAAACGTGAGACCGAACATCATCGACACAGAT
TTTACTCTCGATCAAATGATCGATGCCTTCTCTTCCAAAGGCTTGTCCATTCAAGATCTC
GTCGTCCTCTCAGGTATATATCAACGTTTCTTGAAACTTCTAATCAATTTTACCAACATG
TTTTGCTAAACGCAAACAGAGTATGAAACAGAGTTTTTTTTCTTTAACTATGGTAAAGTC
AATTCTTGTAGCAAAATCTGTTTCATATTCTGTTACACTCAGCTTCTTCTTTTTTTATTT
ACATTCAGGAGCACACACAATTGGAGCATCACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTNCATTCAAAAATGTAGATGTCCG
CAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTAT
TTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGG

Last Updated on 06.12.2018 16:11