Sequences from confirmation amplicon


Confirmation sequences for line 369B07 and hit At2g24990 are shown.
Line ID 369B07
Locus At2g24990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10625804
Primers used for PCR 17ve - 8474, show primer details
   
Sequence Name 52-K117914-0022-369-B07C-17ve
GenBank Accession LN507945 [GenBank]
Sequencing primer gene specific primer, 17ve: 5'-TCCTCCGGTAGTAGAATACGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K117914-0022-369-B07C-17ve
TCTCTCTCTTTATCGGATTCCGATATCGCTGAAGCTCTTGATTGGCTCGACGGAAAAGAC
GATGACGAGCTAATAGGTGGTGGCTTTTCCCTCCATGCTCGTCGTCCTAATGCTCACGGT
GGACATGGTTCACGTCCTAACTCTAGTTCTCTTCAACCTATATCAAATAAAGCCCAGAAG
CTTACTAGTCATGTTCGTGCTTCTCCTTTAGAGGTAACGTTTCTTATCAAATTGATGTTT
TGAATTTGAAAAAGCTTCTGGATTACTTATAAAGATATGATGTTTTGAATTTGAAAAAGC
TTCTGAATTGCTCATAGTCTCTGCTCAAATGAAACATTGTCAAAGCTTCTGAATTGCTTA
TAGGGTTTAAGTTCAGGTAGGTTTTGTACTTGTAAAGTGGAGAACTTTGTGGTATATTGT
AGCTGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAAAAATTATA
   
Sequence Name 12-K117913-0022-369-B07C-8409
GenBank Accession LN491776 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K117913-0022-369-B07C-8409
TATATCCAGCTCAATATACCACAAAGTTCTCCACTTTACAAGTACAAAACCTACCTGAAC
TTAAACCCTATAAGCAATTCAGAAGCTTTGACAATGTTTCATTTGAGCAGAGACTATGAG
CAATTCAGAAGCTTTTTCAAATTCAAAACATCATATCTTTATAAGTAATCCAGAAGCTTT
TTCAAATTCAAAACATCAATTTGATAAGAAACGTTACCTCTAAAGGAGAAGCACGAACAT
GACTAGTAAGCTTCTGGGCTTTATTTGATATAGGTTGAAGAGAACTAGAGTTAGGACGTG
AACCATGTCCACCGTGAGCATTAGGACGACGAGCATGGAGGGAAAAGCCACCACCTATTA
GCTCGTCATCGTCTTTTCCGTCGAGCCAATCAAGAGCTTCAGCGATATCGGAATCCGATG
AAAGAGAGAGATCGTCGTCGTCGTCATCGTCGTGCTCTTCTTCGTATTCTA

Last Updated on 06.12.2018 16:11