Sequences from confirmation amplicon


Confirmation sequences for line 369E06 and hit At4g30290 are shown.
Line ID 369E06
Locus At4g30290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14829753
Primers used for PCR PC84 - 8474, show primer details
   
Sequence Name 14-K103845-0022-369-E06C-PC84
GenBank Accession LN492480 [GenBank]
Sequencing primer gene specific primer, PC84: 5'-GTCCAAACATTATTTCGGTTTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>14-K103845-0022-369-E06C-PC84
TTTTTATTGGATTCCTTGGCAACGCCAACAACGAATCAAATAATCTATATACATAATTAT
ATTAGAAGCTGTGAATGTGCCTCCGAGTAGACAAAAGCACATGCACCATAAATGATCAAA
CCCATGAAACTTCCATCTCTCTTTTTCCCCTATATAAACTAATGTCACTTACCTCAATAT
TCCATCAACAATATACATCTGAGATATCAAAACTCCTAGAGAGATCTAATAAAAATGAAG
TCTTTTACGTTCTTGATTCTCTTTCTCTTTGCAGCACAATCTATCAGCGTCTATGCAGGC
AGCTTCCACAAAGACGTTAAGATACATTGGGGTGATGGTCGTGGAAAGATTCACGATAAT
CAAGGGAAACTTCTATCTCTCTCGCTTGACAAATCCTCTGGTTCAGGTTTCCAATCTAAT
CAAGAGTTTCTCTATGGTAAAGCCGAAATAGATTTCCCGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGAT
   
Sequence Name 30-K103846-0022-369-E06C-8409
GenBank Accession LN499689 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K103846-0022-369-E06C-8409
ATCCGGACTGAGCCTTTACATTGAATATATCCGGGAAATCTATTTCGGCTTTACCATAGA
GAAACTCTTGATTAGATTGGAAACCTGAACCAGAGGATTTGTCAAGCGAGAGAGATAGAA
GTTTCCCTTGATTATCGTGAATCTTTCCACGACCATCACCCCAATGTATCTTAACGTCTT
TGTGGAAGCTGCCTGCATAGACGCTGATAGATTGTGCTGCAAAGAGAAAGAGAATCAAGA
ACGTAAAAGACTTCATTTTTATTAGATCTCTCTAGGAGTTTTGATATCTCAGATGTATAT
TGTTGATGGAATATTGAGGTAAGTGACATTAGTTTATATAGGGGAAAAAGAGAGATGGAA
GTTTCATGGGTTTGATCATTTATGGTGCATGTGCTTTTGTCTACTCGGAGGCACATTCAC
AGCTTCTAATATAATTATGTATATNGATTATTTGATTCGTTGTTGGCTGTTGCCAAGTGA
ATCCAATAAAAAACTCTTTCTTTATTTGTTTATTGTTATCAAACCGAAATAAGNTTTGGA

Last Updated on 06.12.2018 16:11