Sequences from confirmation amplicon


Confirmation sequences for line 369F05 and hit At3g60970 are shown.
Line ID 369F05
Locus At3g60970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22558043
Primers used for PCR KH41 - 8474, show primer details
   
Sequence Name 38-K103228-0022-369-F05C-KH41
GenBank Accession LN502940 [GenBank]
Sequencing primer gene specific primer, KH41: 5'-ATATCTTGCAAAAACATCTGGGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K103228-0022-369-F05C-KH41
CTTTATGGTGATGGCTTGCACTACCCATTGACAAGGCTTCAGAGAAACTACCAATCAGAT
ATCATGAATGCCAAAGATGATAGAATGAAAGCAACTTCAGAGATTCTTAAAAACATGAAG
ATTCTGAAGCTTCAAGCATGGGATAACCAGTTTCTCAACAAGGTTAAAACATTAAGAAAG
AAAGAGTATGATTGTTTATGGAAGTCTTTGAGGTTACAAGATTTCACAACTTTCATACTT
TGGGGAGCTCCTTCATTGATCTCTGTGGTGACTTTTGTTACTTGTATGTTAATGGGAGTG
AAGCTAACAGCTGGAGCAGTCTTATCTGCTCTTGCAACGTTTCAAATGTTACAAAGTCCG
ATTTTCGGGAAACGGCCAATCTGATGGTTTCATGTCCGGGAAATCTACATGTATCAGCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAGA
   
Sequence Name 06-K103229-0022-369-F05C-8409
GenBank Accession LN488769 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K103229-0022-369-F05C-8409
CATCAGATTGGCCGTTTCCCGAAAATCGGACTTTGTAACATTTGAAACGTTGCAAGAGCA
GATAAGACTGCTCCAGCTGTTAGCTTCACTCCCATTAACATACAAGTAACAAAAGTCACC
ACAGAGATCAATGAAGGAGCTCCCCAAAGTATGAAAGTTGTGAAATCTTGTAACCTCAAA
GACTTCCATAAACAATCATACTCTTTCTTTCTTAATGTTTTAACCTTGTTGAGAAACTGG
TTATCCCATGCTTGAAGCTTCAGAATCTTCATGTTTTTAAGAATCTCTGAAGTTGCTTTC
ATTCTATCATCTTTGGCATTCATGATATCTGATTGGTAGTTTCTCTGAAGCCTTGTCAAT
GGGTAGTTGCAAGCCATCACCATTAAAGTTGTGACCAATGCAGCTAANGCTCCNAGTCCC
AGATGTTTTTGCAAGAAA

Last Updated on 06.12.2018 16:11