Sequences from confirmation amplicon


Confirmation sequences for line 370D07 and hit At3g27950 are shown.
Line ID 370D07
Locus At3g27950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10379761
Primers used for PCR BW63 - 8474, show primer details
   
Sequence Name 06-K106136-0022-370-D07C-8409
GenBank Accession LN488820 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K106136-0022-370-D07C-8409
CCATTTACAATTGAATATATCCTGTTGAACATGTCGAAAAAATAAGTTGCCATGATATAA
ACTAATAAATGTCTAGCATATTTCTGGTGCTTTCAAGTAACTAGCCAAAATTTCTATTCA
CATCTCTCTTTTTGTTTTTGAATCAATGTAAAATTTTCTTCAAAAAGTCATTGGTTACAA
TATATGCAGCTTTAAGCAGAATGTTTCTATTCACATCTTATCATATTAGTTATTATATAA
AAGACTATATCAAATAATCAGTAACTTGTTTGGTAAGAAATTTTAAACCGCTTAGTTCAG
ATTATCTTTTGCTTAGTGACAACTTTTCTTTTGTTGCTTGNCTAAGTGA
   
Sequence Name 06-K106135-0022-370-D07C-BW63
GenBank Accession LN488819 [GenBank]
Sequencing primer gene specific primer, BW63: 5'-TCACTTAGCAAAGCAACAAAAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K106135-0022-370-D07C-BW63
CAAGATATCTGAACTAGCGGTTTAAATTTCTTACCAACAAGTTACTGATTATTTGATATA
GTCTTTTATATAATAACTAATATGATAAGATGTGAATAGAAACATTCTGCTTAAAGCTGC
ATATATTGTAACCAATGACTTTTTGAAGAAAATTTTACATTGATTCAAAAACAAAAAGAG
AGATGTGAATAGAAATTTTGGCTAGTTACTTGAAAGCACCAGAAATATGCTAGACATTTA
TTAGTTTATATCATGGCAACTTATTTTTTCGACATGTTCAACAGGATATATTCAATTGTA
AATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGA
GAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG

Last Updated on 06.12.2018 16:11