Sequences from confirmation amplicon


Confirmation sequences for line 370E09 and hit At1g06360 are shown.
Line ID 370E09
Locus At1g06360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1939561
Primers used for PCR BW64 - 8474, show primer details
   
Sequence Name 18-K106020-0022-370-E09C-BW64
GenBank Accession LN494311 [GenBank]
Sequencing primer gene specific primer, BW64: 5'-ATCAATGTGCACTTTTTGTGTCTC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K106020-0022-370-E09C-BW64
GGAGCATTCGGTTCGGTTTCGTGCTCTACGCATTGACTTCACTCAGCATCACTTTCTCAT
ACCATAGGAACTTGGCTCACCGGAGCTTTAAGCTTCCGAAATGGCTTGAATATCCTTTAG
CTTATTTTGCTGTTTTTGCTCTTCAGGTTAATACCGATTAATATTGATAACTATGTATTA
CATATATGTCAATTCCTGTTCGTTTTAGAGTATGATTAGACGATATATTATTTTCAGGGT
GATCCGTTGGATTGGGTGAGCATACATAGGTTCCATCACCAGTTCACAGATTCTGACCGT
GACCCACATAGCCCTATCGAAGGATTTTGGTTCAGTCATGTGTGGTGGATATGTGACACT
CGTTATATCAAATATAAGGTAATGTAGCGAAGAAATGTTGTCTTCTTGTATATAACCTAT
ATATACGATGTTGTAATAATATGCATGTAACAGTGTGGAGGACGTAACAACGTGATGGAC
TTGAAGCAGCAATGGTTCTATTGGTTTCTACGAATGACAATTGGTTTCCACGTCTTAATG
TTTTGGACTGTCCTCTATCTCTATGGTGGTTTACCTTACCTTACATGCGGCGGGGTAAAT
TTATTTCTTCCTATAAACTATATGTATACACACAAAATAATTTGTATGTATCCATATGTA
ACATGATATATGTTATAACAGGGCGTTGGAGGTGTGATTGGGTACCACGTGAACTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCACCGTTAT
   
Sequence Name 18-K106021-0022-370-E09C-8409
GenBank Accession LN494312 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K106021-0022-370-E09C-8409
CTCCACGCCCTGTTATAACATATATCATGTTACATATGGATACCTTAAATTATTTTGTGT
GTATACATATAGTTTATAGGAAGAAATAAATTTACCCCGCCGCATGTAAGGTAAGGTAAA
CCACCATAGAGATAGAGGACAGTCCAAAACATTAAGACGTGGAAACCAATTGTCATTCGT
AGAAACCAATAGAACCATTGCTGCTTCAAGTCCATCACGTTGTTACGTCCTCCACACTGT
TACATGCATATTATTACAACATCGTATATATAGGTTATATACAAGAAGACAACATTTCTT
CGCTACATTACCTTATATTTGATATAACGAGTGTCACATATCCACCACACATGACTGAAC
CAAAATCCTTCGATAGGGCTATGTGGGTCACGGTCAGAATCTGTGAACTGGTGATGGAAC
CTATGTATGCTCACCCAATCCAACGGATCACCCTGAAAATAATATATCGTCTAATCATAC
TCTAAAACGAACAGGAATTGACATATATGTAATACATAGTTATCAATATTAATCGGTATT
AACCTGAAGAGCAAAAACAGCAAAATAAGCTAAAGGATATTCAAGCCATTTCGGAAGCTT
AAAGCTCCGGTGAGCCAAGTTCCTATGGTATGAGAATGTGATGCTGAGTGAAGTCAATGC
GTAGAGCACGAAACCGAACCGTAATGCTTCCCATTTGTAGTTAAATGGTGCCAAGAGACA
CAAAAAGTGCACATTGA

Last Updated on 06.12.2018 16:11