Sequences from confirmation amplicon


Confirmation sequences for line 370G06 and hit At2g39280 are shown.
Line ID 370G06
Locus At2g39280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16406067
Primers used for PCR 19uj - 8474, show primer details
   
Sequence Name 23-K117356-0022-370-G06C-19uj
GenBank Accession LN496725 [GenBank]
Sequencing primer gene specific primer, 19uj: 5'-TCTTTCAACTATCACCGCCTCTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K117356-0022-370-G06C-19uj
ATCGCCGACGCCGTTTCTAGTCTGACCACAAAAGGTATCGCCTTTCGAGCTTCTCCATTG
CTACATTGCTTATCTCGTTGTGTTTTTGTTTCTAATTTACTACATTTGATGGAATTTGTT
TTTTGAAATTGCGGTTTCTCAGTTAATCTCTCTGCTCTCGATTTGCTGAGCAAAATGTTG
TCTTTTTAGATCACTAGTTGAGAATCTAGTGATCATTGATCTTGATTTCATCTATATTCT
TAGTACTTAGGGAAGTTAATGAGGTTGGTTTTATGAACAGGGATGCATATGGATTTTCTG
TGAGGCCTCAGCATGTGCAAAGATACCGAGAATATGTTAATATCTACAAGGTTCTTTCTA
TCTCCATAACTTTTATTTTCTACTCGGAAAATTCTTTTACATGATTGAGTGCACACTGGC
ATTCTTAGGAAAAAAGGAAGGTTTGTCTAAAATTTGGTGATCCATTAAAGTAGCTTTGTT
ACAGCTGTGTGAAAGATTTCAGAAGTGGACTTAACTTAGAAGTTTACTTAGTGGTCTTGG
TTTAATCAATATCGAAAGGCTTCATATATCATAAGTCATTATGGCTTCATGTCAGGATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCACGGTT
   
Sequence Name 03-K117355-0022-370-G06C-8409
GenBank Accession LN487658 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>03-K117355-0022-370-G06C-8409
TTGAATATATCCTGACATGAAGCCATAATGACTTATGATATATGAAGCCTTTCGATATTG
ATTAAACCAAGACCACTAAGTAAACTTCTAAGTTAAGTCCACTTCTGAAATCTTTCACAC
AGCTGTAACAAAGCTACTTTAATGGATCACCAAATTTTAGACAAACCTTCCTTTTTTCCT
AAGAATGCCAGTGTGCACTCAATCATGTAAAAGAATTTTCCGAGTAGAAAATAAAAGTTA
TGGAGATAGAAAGAACCTTGTAGATATTAACATATTCTCGGTATCTTTGCACATGCTGAG
GCCTCACAGAAAATCCATATGCATCCCTGTTCATAAAACCAACCTCATTAACTTCCCTAA
GTACTAAGAATATAGATGAAATCAAGATCAATGATCACTAGATTCTCAACTAGTGATCTA
AAAAGACAACATTTTGCTCAGCAAATCGAGAGCAGAGAGATTAACTGAGAAACCGCAATT
TCAAAAAACAAATTCCATCAAATGTAGTAAATTAGAAACAAAAACACAACGAGATAAGCA
ATGTAGCAATGGAGAAGCTCGAAAGGCGATACCTTTTGTGGTCAGACTTAGAAACGGCGT
CGGCGATCATAATTCCTTAGTTGGAGAACGAGATCTCTCCGAATAGAGGCGGT

Last Updated on 06.12.2018 16:11