Sequences from confirmation amplicon


Confirmation sequences for line 371C10 and hit At3g18570 are shown.
Line ID 371C10
Locus At3g18570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6396391
Primers used for PCR LL05 - 8474, show primer details
   
Sequence Name 49-K022913-022-371-C10C-8409
GenBank Accession LN506697 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K022913-022-371-C10C-8409
CATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATCCTCCTTCTTGGTGTA
AGCGTCACCGCTGCCGTGCTCGGCTTCATTGTCTTTCTTCCATTGATCATCATCTCGAGT
CCGATATGGATTCCCGTTTTTGTCGTCGTTGGCGGGTTCTTAACGGTCTCCGGATTTCTT
GTCGGAACGGTGGCTTTAGTGTCGTGGACGTACCGCTATTTCCGGGGAATGCACCCGGTT
GGATCGAATCAGATGGATTATGCACGTAGTAGGATCTATGACACGGCATCTCACGTCAAA
GATTACGCTAGAGAATACGGTGGTTACTTCCATGGCAGGGCTAAAGATGCGGCCCCTGGT
GCTTGACCTGAACCGGTTGGTATAGTCTGGTTCAGGGTAGTATAAACCGA
   
Sequence Name 53-K022913-022-371-C10C-LL05
GenBank Accession LN508016 [GenBank]
Sequencing primer gene specific primer, LL05: 5'-ATCGGTTAATACTACCCTGAACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K022913-022-371-C10C-LL05
TATACCAACCGGTTCAGGTCAAGCACCAGGGGCCGCATCTTTAGCCCTGCCATGGAAGTA
ACCACCGTATTCTCTAGCGTAATCTTTGACGTGAGATGCCGTGTCATAGATCCTACTACG
TGCATAATCCATCTGATTCGATCCAACCGGGTGCATTCCCCGGAAATAGCGGTACGTCCA
CGACACTAAAGCCACCGTTCCGACAAGAAATCCGGAGACCGTTAAGAACCCGCCAACGAC
GACAAAAACGGGAATCCATATCGGACTCGAGATGATGATCAATGGAAGAAAGACAATGAA
GCCGAGCACGGCAGCGGTGACGCTTACACCAAGAAGGAGGATATATTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
TAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAA
AGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11