Sequences from confirmation amplicon


Confirmation sequences for line 371H06 and hit At5g28540 are shown.
Line ID 371H06
Locus At5g28540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:10541202
Primers used for PCR BJ80 - 8474, show primer details
   
Sequence Name 12-K104856-0022-371-H06C-BJ80
GenBank Accession LN491568 [GenBank]
Sequencing primer gene specific primer, BJ80: 5'-ATCTTCTCCTTTTCATCTCCTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>12-K104856-0022-371-H06C-BJ80
GTTGTACCGTATGTCTCCAGGGCATTCCTGGCGTCGATCTTCTCCTTTACCTTCTTGTCT
TCCTCTGCAAACTCCTCTGCCTCCTTCACCATCCGGTCAATCTCTTCTTGGCTCAGACGA
CCCTTCTCGTTTGTGATAGTAATCTTCTCTGATTTACCACTCGCCTTGTCCTCTGCTTTC
ACATTAAGAATACCGTTGGCGTCCACTTCGAATGTGACCTCGATTTGAGGGGTTCCTCTG
TCAAAACGAGATCAGGATAATTATAAATATGGTCAATTGTAAATATGGCCTCATGATATA
TTCAATATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCG
   
Sequence Name 28-K104857-0022-371-H06C-8409
GenBank Accession LN498837 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K104857-0022-371-H06C-8409
GCCTTTCATATTGAATATATCATGAGGCCATATTTACAATTGACCATATTTATAATTATC
CTGATCTCGTTTTGACAGAGGAACCCCTCAAATCGAGGTCACATTCGAAGTGGACGCCAA
CGGTATTCTTAATGTGAAAGCAGAGGACAAGGCGAGTGGTAAATCAGAGAAGATTACTAT
CACAAACGAGAAGGGTCGTCTGAGCCAAGAAGAGATTGACCGGATGGTGAAGGAGGCAGA
GGAGTTTGCAGAGGAAGACAAGAAGGTAAAGGAGAAGATCGACGCCAGGAATGCCCTGGA
GACATACGTGTACAACATGAAGAACCAAGTGAACGACAAGGACAAGCTTGCCGACAAATT
GGAAGGAGATGAAAG

Last Updated on 06.12.2018 16:11