Sequences from confirmation amplicon


Confirmation sequences for line 372A02 and hit At3g22890 are shown.
Line ID 372A02
Locus At3g22890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8113105
Primers used for PCR HF18 - 8474, show primer details
   
Sequence Name 44-K032417-022-372-A02C-8409
GenBank Accession LN505076 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K032417-022-372-A02C-8409
CTTTCTCATTGCAAGTCAATCGCCGTCAGCTCAACACGTGGCAAATCCGCCGCCTCGTGT
TTCTTCTCTCGCCGCTTTGGCTCTTCCACGATGAGCTCTACAAGCTTACCACCGTCGGGA
GCGATTAATCCGGCTCGGATTGATCCTACGCGGCGGCGTAGGGATTTGGAAGGGAAGGAA
ACCGCGGCGAAGGGGAGATCGGAGTTTGGAGATGATTTGGTTAGTGGTTGAGAGAGGAAT
GGAGTTTTGCTTAAGACGGCAGCCATTGAAGCCATTGTTGAAGGTTTTGTTAGGCTATGG
CTGCTTCAAGCTCTAATGGAGAGAGATAGAGATTTGGTTTGGTTCCCA
   
Sequence Name 44-K032417-022-372-A02C-HF18
GenBank Accession LN505077 [GenBank]
Sequencing primer gene specific primer, HF18: 5'-TTGGGAACAAACCAAATCTCTATC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K032417-022-372-A02C-HF18
CATAGCCTAACAAACCTTCACAATGGCTTCAATGGCTGCCGTCTTAAGCAAAACTCCATT
CCTCTCTCAACCACTAACCAAATCATCTCCAAACTCCGATCTCCCCTTCGCCGCGGTTTC
CTTCCCTTCCAAATCCCTACGCCGCCGCGTAGGATCAATCCGAGCCGGATTAATCGCTCC
CGACGGTGGTAAGCTTGTAGAGCTCATCGTGGAAGAGCCAAAGCGGCGAGAGAAGAAACA
CGAGGCGGCGGATTTGCCACGTGTTGAGCTGACGGCGATTGACTTGCAATGAGTAAATGG
CTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAA
AGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTA
TAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAA
AGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCA

Last Updated on 06.12.2018 16:11