Sequences from confirmation amplicon


Confirmation sequences for line 372H02 and hit At1g06640 are shown.
Line ID 372H02
Locus At1g06640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2033006
Primers used for PCR 33nt - 8474, show primer details
   
Sequence Name 55-K114626-0022-372-H02C-33nt
GenBank Accession LN508887 [GenBank]
Sequencing primer gene specific primer, 33nt: 5'-GTACACTCTAGGATTTGGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K114626-0022-372-H02C-33nt
TGGCTCTGTTCGCCATACTCTATGCTTCAACTGATGAACTTGTCGTTTGTTATAAGCTGC
AGCCAACGCAATTAAAAAAAAAAAAAACCTTTGACCCAGGGGGAAAATTTCTTAACCAAA
TCTGCCATTGAAAAAAACCTGGGGAAACCGGACCTACCAAACTGGTTTGGTTGGCCCAAA
CTTGCCGGCAAAAAATCCCCAATTTTAATAAAAAAACCCCCGGGAACTTGAGGAACTTCA
AACCTTTTCCCCTCACAACAAACCGGAACCCCTTCTTTTTTATCGGGAAAAAGGACGGTA
AAAAAAAACCCCCCGGAGGGCTTACTTGGCCCAAAATTTAGGTCGGGCCCCGGACCCGGG
GGGAATTAGGGGCAAACCAGGCCCAACCCCTTTAACCCCCCCGGGCCCTTGGGGGGATAG
GGGTTCACCCCTGGGGCTTCTAACAAAACCTCAAACAAAATTTCCCCCAAAACCCCCATT
GGCTTGGAGTATCCCCCCAAAACCCCCCTAAAAAAACCCCCCAAAAACAAAAAAGGAGAT
TTAAAATTTGCTAACGTGTCCACAAGGGCTCCATTGGAAAAGGGCTCCAGGCCCGGGAAA
TCCCCGGGGATCCCCATGGAGTATAAGGGTCAATAGGGAAAAAAAAAAAGAGTATTTCCC
ATTTTTTTTTCATTTCAAAAAGGAAAATT
   
Sequence Name 55-K114625-0022-372-H02C-8409
GenBank Accession LN508886 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K114625-0022-372-H02C-8409
TTGAAGCCTTTGTGAACACGTAAGCAACTTTCATATCTCTTTATTCGTTCTTGGTGGATT
CTTTCAGGGATGTTATGATGGAATACTCAAAGCAAGTGATGATTTTGGGTGAATTTCTGT
TTGAGCTTTTGTCAGAAGCACTAGGGTTGAACCCTAATCACCTCAAGGACATGGAGTGCT
TAAAGGGTTTGCGCATGCTTTGCCACTACTTCCCACCGTGTCCGGAGCCTGACCTAACTT
TTGGCACAAGTAAGCACTCCGACGGCTCTTTTCTTACCGTCCTTCTACCAGATAACATAG
AAGGGCTTCAGGTTTGTCGTGAGGGATACTGGTTCGATGTTCCTCATGTTCCCGGCGCTC
TCATCATCAACATCGGAGATCTTCTGCAGGCAAGTTTGCGCAATCAATACATGTTTGCTA
GCTCAGATTTCACAAGTTTATTTACAATGCTAGATTTGTTTAAGAAATCTTCCTCTTGGT
TCAAAGTTTTTTTTTTTTTTAAATGGCGTGGGCGGCCCCTAAAAAAAAACAACAATTTCA
TCTTTTTAAACCAAAAATTTTGGGCAAAAAAACCCCCAAAACCTCGATTCTCTTTCCAAG
TTTTCTCCCAACCCCCCTTAAAACCAA

Last Updated on 06.12.2018 16:11