Sequences from confirmation amplicon


Confirmation sequences for line 373A07 and hit At1g60730 are shown.
Line ID 373A07
Locus At1g60730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22360054
Primers used for PCR BJ38 - 8474, show primer details
   
Sequence Name 07-K104372-0022-373-A07C-BJ38
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, BJ38: 5'-GTTGCTCAATTAAGATATGCATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K104372-0022-373-A07C-BJ38
AGTTACTTCCAGACTCAAGCCAAAAAAAAAAGGGTTATTTCCAAAACTTGAAATTGGATT
TTTTTTTATTTCCAAAACCAAATGGTTATCCACCACCTGGCGGCGTTTTTTATAAAAAAT
AATTCCAAAAATATTTCTGGAAACCAAAACCTAAAAAAAAAAATTGGTCCTCCAATTTTC
CACCCAAAAAAACCTTTTATCCTTGACCAATTTTTTGGAAAGTTTTTTTTCCCCCAAAAG
TTTAAAATTTGTTGGAAAAAAAAGGGGAAAACTTTTTTGGGTAACCTCCTCCAAAAAGGA
TTTGGACTTAGAAAAATCCCTTTCCTTTTTTGGGTAAACAATTCCATTTTTTTTTCCTTT
AAAAGGAAAAAAAAAACCGCCCCCCCCCCCCCAAATTGACCCCGAATTTTTTTTCCCCCT
CCACCCAATCCAACCCCCTTTAAATTCCCCGCCTTTTTAAAATCCCCCAAACCATTCCAA
AAAAAAAAAATTTTTTAAATTTCAAACAAAACCCCGGGGGGAACCCTGGCGAATGAAAAA
GAAAAAAATTTCCCGGGTTCCATTGGAAATGGCCTCCTGGCCGGGAAATCCTCCGGGGTC
CCCCATGGATTAGAAGGGCCAATTGGGAAAAAAAAAAGAATTATTCCCCATTTTTTTTCC
ATTCCAAAAGGGAAATTTCCCCCC
   
Sequence Name 23-K104373-0022-373-A07C-8409
GenBank Accession LN496540 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K104373-0022-373-A07C-8409
CATTTACAATTGATACCGGGAATATATTATCCTTCTCACTCCGCAAGCTTCCGCCATGAG
TCTCTGTCAGAAGTCAGAACTATCTCTCTCTCTTGACTTGTCTAGGTGACTCTTATAGAG
CTGTGAATTTAAATGGAGTTGGATTGGTGTGATGGTGAAGATAAATTCAGTGTCAATTTT
GGATGGCTGGTGCAGTTTTTTTTTTCCCTCTTAACGAAAACAAAATTGAATTTGTTAACC
AAATAAAGTAATGGAATCTTCATAAGTCAAACTCATTTTTGATGTATTTAACCAAATAAT
GTCTTCACTTTCTTTCTCAATCAATTATTAACTTCTTGTGAAAAATAAACTTTCCTAATA
ATTTGTTCACGTATAAATGTTTTTTTAGGTTGAAAATTTGAAGTACAATTTTCTCTTATA
AGTTTTTGTTTTCAAAATATATCTTTGAATTAATATTATATAAAAACGCACCAGGTAGTA
TTATAACATTTTGATCTTGAAAATAAAAAAATATCATTTTCAAAGTCTTGAAAATAACCT
TTTTTTTTTTGGCCTAAAATTTTGGGAAATTAACTTGGGAAACCCCCCACCCCCGAGGCC
ATTTTTA

Last Updated on 06.12.2018 16:11