Sequences from confirmation amplicon


Confirmation sequences for line 373B06 and hit At1g04180 are shown.
Line ID 373B06
Locus At1g04180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:1105033
Primers used for PCR MM68 - 8474, show primer details
   
Sequence Name 42-K020358-022-373-B06C-8409
GenBank Accession LN504305 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K020358-022-373-B06C-8409
GATCCATGTAGATTTCCCGGACATGAAGCCATTTACAATTGAGGTTGTTCTAACCCTCCA
TAGCCCGCTAGTTTCATCAAACCGAGCAGACTCAACGCTTTTATTAAACTCCGGTTTAAT
ATCGAACCGGTTTGCGTATGACTCAAGGTAATCGATGAACTGTCGTTTCGTTGGGTATTC
AGGGTAGTGATCCGGGAAGGGCATTTTCGGTAATTGGCAGAATTTCTTGGGCAAGTGAAG
CTTGAGCCTATCGTAGGTTCGTTTTTGCCACAGTGACGCTATGCAATCTGATCTCTCGAC
CACAACGAATGGGACTCCTTGATCATGTAGACAAGCTGCTGTAGCTAAACCGGATGGACC
AGCTCCTACGATAACCGGACCGTTGACCCAAACACATCGCCGCTCTGAGAAATATTCTTC
ACTTGCCATGAGTCTAAACATATTCTCCATTTTCTTGAGTGAGTTTTTGAATGAAAGCAA
GTATATGTTTTGAGGGTGGGTTTTGATCGATTTGAAAGTATATGTAGTGAAGTTGAATGT
TAGTTTTTGTTGTATTATGAGAAATGAGATCTATTGGTTT
   
Sequence Name 46-K020358-022-373-B06C-MM68
GenBank Accession LN505726 [GenBank]
Sequencing primer gene specific primer, MM68: 5'-CACTCCAAATCCAAACCAATAGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K020358-022-373-B06C-MM68
TTTCTCATAATACAACAAAAACTAACATTCAACTTCACTACATATACTTTCAAATCGATC
AAAACCCACCCTCAAAACATATACTTGCTTTCATTCAAAAACTCACTCAAGAAAATGGAG
AATATGTTTAGACTCATGGCAAGTGAAGAATATTTCTCAGAGCGGCGATGTGTTTGGGTC
AACGGTCCGGTTATCGTAGGAGCTGGTCCATCCGGTTTAGCTACAGCAGCTTGTCTACAT
GATCAAGGAGTCCCATTCGTTGTGGTCGAGAGATCAGATTGCATAGCGTCACTGTGGCAA
AAACGAACCTACGATAGGCTCAAGCTTCACTTGCCCAAGAAATTCTGCCAATTACCGAAA
ATGCCCTTCCCGGATCACTACCCTGAATACCCAACGAAACGACAGTTCATCGATTACCTT
GAGTCATACGCAAACCGGTTCGATATTAAACCGGAGTTTAATAAAAGCGTTGAGTCTGCT
CGGTTTGATGAAACTAGCGGGCTATGGAGGGTTAGAACAACCTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCT

Last Updated on 06.12.2018 16:11