Sequences from confirmation amplicon


Confirmation sequences for line 373G02 and hit At1g24070 are shown.
Line ID 373G02
Locus At1g24070 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8518721
Primers used for PCR 59yx - 8474, show primer details
   
Sequence Name 52-K123255-0022-373-G02C-59yx
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 59yx: 5'-ATTGAGAAAAGGACCTTATTGCG-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K123255-0022-373-G02C-59yx
CTGGACCACAAGACCATCGATGTTGCTGGAATCTAAAGGCCTTAAACTTGCTTGGTAACT
CGCTTTTCACCTACAAACTCAGTGTTCATATACATAATGTAATGATTATAATTTAGTAAT
TATTATTTTATAGCAATGCATCACTATATATATATATAGAATTATAGATATCATTGCACA
TCAAGACAAGAGTGCCACACTAGTCAGAAACTATGTCCATAACCAAATTCTATAAAATCT
ACCACAATAACTATAGTTCAAAAGGGGAGTTTCTATGTGACTTTAATAAAGTTATAGTTT
CAAAAGTGGAAGCATACAAAAAAAAAGTGGGAGTGTCTATGTAAAGAAAGCACTAGTAAA
TGTTGGATATTAAATCCACATGGGAACAGTTACATAATGTACGAAAGATGCAATGAGGGC
TTATTTACAGCAATTAATAGGTAGGGATAAAATCAAGTACCGTGAGGTCATTGAGAAAGA
CAAATTTCCAGCCGAGAAGACCAGCACGAACGGCCAAGTCCATGTCCTCTACAGTAGTCC
GGTCATGCCATCCTCCAGCTTCTTCCATCGCGGCCATTCTCCATACACCCGCAGTTCCTG
AAAGAGATATACCAAAACCATATTTATAAAATAAGTACCTAAAATTTATCAAAAATTCAA
AACATGTAATGATAACCAAATACACCATTGAAGCTAAAGAAGGCATGTCTAGTGGATCCA
GATTCTTGCTCTGCCATAAAGTGGTAGTTGAGGGACATCTCTTGCATCCTCGTCATCAAG
CACTTGTTTGCGTTCACTGCAATGAACCCCACACACACATACAATCACTCAGTTTGAAAA
TAAACTTTGTTAGGATCTTTATTTATGTACATAGCATTGTTACGAAAGATATAAGTTCAG
TTTAT
   
Sequence Name 20-K123254-0022-373-G02C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K123254-0022-373-G02C-8409
ACTTCCAACCGGAGCCTGATACCTCCACACTCTGTCCCCTTCCTCGTTCATAACCCCGAG
GTCGCTCTGGTTCAAGCCCGATGGAGATTCAGTACGTACATTATGGTAGACAATTTTACG
TACCTAAACATTTACAACTATTAACTTATATGAGACTATCAATATTTGTAAATTTTTACT
CCATATCTAGGTTGTGATAAACTGAACTTATATCTTTCGTAACAATGCTATGTACATAAT
TAAGGATCCTAACAAAGTTTATTTTCAAACTGAGTGATTGTATGTGTGTGTGGGGTTCAT
TGCAGTGAACGCAAACAAGTGCTTGATGACGAGGATGCAAGAGATGTCCCTCAACTACCA
CTTTATGGCAGAGCAAGAATCTGGATCCACTAGACATGCCTTCTTTAGCTTCAATGGTGT
ATTTGGTTATCATTACATGTTTTGAATTTTTGATAAATTTTAGGTACTTATTTTATAAAT
ATGGTTTTGGTATATCTCTTTCAGGAACTGCGGGTGTATGGAGAATGGCCGCGATGGAAG
AAGCTGGAGGATGGCATGACCGGACTACTGTAGAGGACATGGACTTGGCCGTTCGTGCTG
GTCTTCTCGGCTGGAAATTTGTCTTTCTCAATGACCTCACGGTACTTGATTTTATCCCTA
CCTATTAATTGCTGTAAATAAGCCCTCATTGCATCTTTCGTACATTATGTAACTGTTCCC
ATGTGGATTTAATATCCAACATTTACTAGTGCTTTCTTTACATAGACACTCCCACTTTTT
TTTTGTATGCTTCCACTTTTGAAACTATAACTTTATTAAAGTCACATAGAAACTCCCCTT
TTGAACTATAGTTATTGTGGTAGATTTTATAGAATTTGGTTATGGACATAGTTTCTGACT
AGTGTGGCACTCT

Last Updated on 06.12.2018 16:11