Sequences from confirmation amplicon


Confirmation sequences for line 374E12 and hit At4g20450 are shown.
Line ID 374E12
Locus At4g20450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11026451
Primers used for PCR EQ40 - 8474, show primer details
   
Sequence Name 18-K103006-0022-374-E12C-8409
GenBank Accession LN494259 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K103006-0022-374-E12C-8409
TTACATTGAATACCGCATTTACTCGGGTATAAATGGCTTACTCCAGCTTCTTCAGACCGA
AACAGATGAAAACGACGGTTTGTCAACTGTACTTAATTACTATGTAGATATACGAACCCT
TTTTGGTTGTTCATGAGGTTAAACTATTCAACAACATATAAACTTTATATTCCCAGTCAC
TACATTGAAGAACATTCAAGCCACTTATAGAATACAAAAAACGAACTGGCAAGGAGATCC
ATGTGTACCTATACNGTTTATATGGACTGGCCTAAACTGTAGCAACATGTTTCCGTCTAT
TCCACCAAGAATCACTTCCATGTAAGATCATTACACTACTCANGTTTTCTTGTTTAAATC
AATTCTTTTTTTCTTTCTTTTTAATGGAAATTTAAATGTGCCCTGGTCTATATGCAGAGA
CTTTTCTAACTTTGGATTAATTGGGACC
   
Sequence Name 66-K103007-0022-374-E12C-EQ40
GenBank Accession LN511581 [GenBank]
Sequencing primer gene specific primer, EQ40: 5'-TGGTCCCATTTAATCCAAAGTTAG-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K103007-0022-374-E12C-EQ40
AATTTCCTTAAAAGAAAGAAAAAAAGAATTGATTTAAACAAGAAAACTTGAGTAGTGTAA
TGATCTTACATGGAAGTGATTCTTGGTGGAATAGACGGAAACATGTTGCTACAGTTTAGG
CCAGTCCATATAAACTGTATAGGTACACATGGATCTCCTTGCCAGTTCGTTTTTTGTATT
CTATAAGTGGCTTGAATGTTCTTCAATGTAGTGACTGGGAATATAAAGTTTATATGTTGT
TGAATAGTTTAACCTCATGAACAACCAAAAAGGGTTCGTATATCTACATAGTAATTAAGT
ACAGTTGACAAACCGTCGTTTTCATCTGTTTCGGTCTGAAGAAGCTGGAGTAAGCCATTT
ATACCCGAGTAAATGCGGTAATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAA
AACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATT
CGGAAATCTTTATTTCGACGTGTCTACATTCACGTCCAAATGGGACC

Last Updated on 06.12.2018 16:11