Sequences from confirmation amplicon


Confirmation sequences for line 374F11 and hit At5g24060 are shown.
Line ID 374F11
Locus At5g24060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8131507
Primers used for PCR 41uv - 8474, show primer details
   
Sequence Name 73-K117997-0022-374-F11C-41uv
GenBank Accession LN512968 [GenBank]
Sequencing primer gene specific primer, 41uv: 5'-ATTCTGCTGCATGGTGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K117997-0022-374-F11C-41uv
GGAGATATAACTGTGATAAGTGACATTTGCAATGCAGCAAGTAATAGTAGGTTTTGATAC
GATGGAGATGATCAAGGACATGGAGCTGAGCAGTCCATCTGGTATTGGTTTTGGGATTGA
AGAAATCGAAGATGGTGAAAGTGAAGTTGAGGATGAAAACAAGGGCGATGAGGATGAAGG
AGAAGACAAAGATGACGAGGTTTGAAAAGCTTTCGTGGTCTTATTGAGGAACTTCACATG
GAAAAAACCTGTCTTATGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCACGATTATTAT
   
Sequence Name 33-K117996-0022-374-F11C-8409
GenBank Accession LN501174 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K117996-0022-374-F11C-8409
TTTCACCATCTTCGATTTCTTCAATCCCGAAACCAATACCAGACGGTACTGCTCAGCTCC
TTGTGCTTGATCATAACCATTATATAAAAACCTACTATTACGTTGCCGCGTTGCAAATGT
CACACATCACAGGTATATCTCCATACCTATGAATAGAGAACAGAACAAAAGCTGCTGTAA
AGAAAACA

Last Updated on 06.12.2018 16:11