Sequences from confirmation amplicon


Confirmation sequences for line 376B06 and hit At1g11080 are shown.
Line ID 376B06
Locus At1g11080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3695567
Primers used for PCR BW72 - 8474, show primer details
   
Sequence Name 23-K106020-0022-376-B06C-BW72
GenBank Accession LN496560 [GenBank]
Sequencing primer gene specific primer, BW72: 5'-CAGAAGTTCTCAAGCAGTATCACG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K106020-0022-376-B06C-BW72
CTCAGTTTGCTAGGAGATTCTGCGCGATCGTCTTACTTTGATTCAGCGCAATTTAAACAA
ATTCTCGCATTAGCTCCAAGAGGGTATGTGCATGTGTATAACATAAAAGCCTATAATTTA
CTTTGAAACTAACAAATATCTTTTATTTTGTTGGGATGCCTTAGATGCCACCGAGGCTCA
TGGGTGGATATGATCCATGCTTAGATGATTACGCGAGAGTATTTTACAACAGAGCAGATG
TTCAGAAGTCTCTTCATGCGAGTGATGGAGTTAATCTCAAGAACTGGAGCATTTGCAAGT
ACTCTCCCCAATATCCTTTATGGTTTTTTTTTTGTGTAATATTTGAAGGAATTTGTCTGA
ATGTTCTTTGGTATTCTAACTTCAGCATGGAGATCTTTAATAATTGGACTGGTTCAAACC
CGTCGGTTTTGCCTACACTGATAGTCAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCACCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAAATGTCCGC
   
Sequence Name 23-K106021-0022-376-B06C-8409
GenBank Accession LN496561 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K106021-0022-376-B06C-8409
TTACAATTGAATATATCCTGACTATCAGTGTAGGCAAAACCGACGGGTTTGAACCAGTCC
AATTATTAAAGATCTCCATGCTGAAGTTAGAATACCAAAGAACATTCAGACAAATTCCTT
CAAATATTACACAAAAAAAAAACCATAAAGGATATTGGGGAGAGTACTTGCAAATGCTCC
AGTTCTTGAGATTAACTCCATCACTCGCATGAAGAGACTTCTGAACATCTGCTCTGTTGT
AAAATACTCTCGCGTAATCATCTAAGCATGGATCATATCCACCCATGAGCCTCGGTGGCA
TCTAAGGCATCCCAACAAAATAAAAGATATTTGTTAGTTTCAAAGTAAATTATAGGCTTT
TATGTTATACACATGCACATACCCTCTTGGAGCTAATGCGAGAATTTGTTTTGAATTGCG
CTGAATCAAAGTAAGACAATCGCGCAAAATCTCCTATGCAAACTGAGGTATAGATGCTAT
AGATATCTATCTCGTGATACTGCTTGAAAACTTCGG

Last Updated on 06.12.2018 16:11