Sequences from confirmation amplicon


Confirmation sequences for line 376C09 and hit At1g55290 are shown.
Line ID 376C09
Locus At1g55290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20626213
Primers used for PCR PE50 - 8474, show primer details
   
Sequence Name 21-K022565-022-376-C09C-8409
GenBank Accession LN495489 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K022565-022-376-C09C-8409
ATGTCCCGGACATGAAGCCATTTACAATTAAATATATCCCGGACATCTATTTGATTTCTT
CTTGTTTTCATCTAAAGAGTTCTTGTACTAAAAAGATTCTTGCTAATATGTTACTTTGGT
GTAACTAAGCTTAAGGTTAAATTTCGGTTTGTTTCGATGATTTTGGTTTCTCTGTTATTA
CTAGTAGGCAGCCCGTGCTTTGCACGGGAAAATAACAAATACCAATAGTATAGTATTTTG
TTTCTTTGAAATCTGCAACTATTACACTAATGATGAATGTCACAGCCTCACAAAATACAA
TTTGTTTAGTATTATTTTATCGAAATACATTGATTGGATGTATTACACATTGCTCTGTAA
CTCTCAATAGAAGTCTTTCTCAACTTCACCCAAATCGTGATATATATATATATATATATA
TATATATATATATATGTGTGTGTGTGTCAAAAACAAAGACTCTTTTTTTAGACACAACAG
AGTCTCTTATATATACACACTCTCTGTCTCACACATAGAAAAAAAAAAAGAGAGAGTGTA
TATAAAAAAAACAAAAGATATACTGAGCTTTTTTTTCTTCTCCAAAAGCTTAAAAAAAAT
AGTGGGGGAGA
   
Sequence Name 22-K022565-022-376-C09C-PE50
GenBank Accession LN495937 [GenBank]
Sequencing primer gene specific primer, PE50: 5'-CGTTTTGATTCGAAGGTTATTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K022565-022-376-C09C-PE50
TCCATCATTATTATTGTTAAGCTTTTGGAGAAGAAAAAATAGCTCAGTATATCTTTTGTT
TTCTTTATCTACTCTTTCTCTTTTTTTTTTTCTATGTTTGAGACAGTTAGGGTATTTATG
TAAAATACTTTGTTGTTTCTAATGAAAGTTTCTTTGATTTTGACACACACATACATATAT
ATATATATATATATATATATATATATATATCACTATGTGGGGGAAAAAAAAAAAAATTTT
TTTTGAGAGACACAGAAAAGTGTGAAACACACCCAATCTGTGTTTTTCGAAAAAAAAATA
CAAAACAATTTTTTTTTTGGGGGGGGGTGACATTCCTCTTTAGGGAAATAGGGGCATATT
TCAAAAAAAAAAAAAACTATACTGGGGGTGTTTTTTTTTTCCCCGCGAAAAACGGGGGGT
GTCTTTTTTTTAAAACAGAAAAACAAATTTTTTCGAAACAAACAGAAATTTCCCCTTAAG
CTTTGCCCCCCCAAAGAAATATTTTAGAGAGTTTTTTT

Last Updated on 06.12.2018 16:11