Sequences from confirmation amplicon


Confirmation sequences for line 377A01 and hit At3g25180 are shown.
Line ID 377A01
Locus At3g25180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9168212
Primers used for PCR KP77 - 8474, show primer details
   
Sequence Name 09-K034064-022-377-A01C-8409
GenBank Accession LN490078 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K034064-022-377-A01C-8409
TGAAGCCATTTCAACCATGATTGAATTAAGTCATATATATTTCTCATATAAACACTACAT
TAGTTCATAAATTATTTGTTTGTGAGATTTTCTAACATATATATCCAAATTAACGAGAGG
TGAAAATTATATGTGCATACCAAAATTGTTGCCTTCACAATGACATCGCGTACGTGTCCA
CTTATCACAATATCCTCTGGTAAGATGTCTAGCAGTAGATCCATGATTGTTCTTTCTTGA
TCCTTCTCATTTCTTGATCTCTTCTTGAGATGTTCGTGGAGCCACTTGGTGTTGACTGAG
TCAAGCTCCTTAAATAGTCTCTTCATTTGAGAATTTTTTGCAAAATCCAACCATCCCAAC
CATGGAATAACGTCGCCTATCATAGGAATCACAGCCAAGTACTCGCAATGCTTCAGGGCC
TCCTTATAACGCCATTCATCGCTATTCACTTCACCGAAACCAATCCTCTTCCCCACCATC
TTCCTAAGGATTATATTGAAGGTCAAAAACTCAAATAACATGTCAATCTTCACTATAGAA
GTTCCACCATTCCCTTTGTATAGGTGTTTGATCAACGTGTTTACTTCTGAAGAACGAATG
TGACC
   
Sequence Name 76-K034064-022-377-A01C-KP77
GenBank Accession LN513336 [GenBank]
Sequencing primer gene specific primer, KP77: 5'-TATAGAGATGCTTGGTCACATTCG-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K034064-022-377-A01C-KP77
AACACCTATACAAAGGGAATGGTGGAACTTCTATAGTGAAGATTGACATGTTATTTGAGT
TTTTGACCTTCAATATAATCCTTAGGAAGATGGTGGGGAAGAGGATTGGTTTCGGTGAAG
TGAATAGCGATGAATGGCGTTATAAGGAGGCCCTGAAGCATTGCGAGTACTTGGCTGTGA
TTCCTATGATAGGCGACGTTATTCCATGGTTGGGATGGTTGGATTTTGCAAAAAATTCTC
AAATGAAGAGACTATTTAAGGAGCTTGACTCAGTCAACACCAAGTGGCTCCACGAACATC
TCAAGAAGAGATCAAGAAATGAGAAGGATCAAGAAAGAACAATCATGGATCTACTGCTAG
ACATCTTACCAGAGGATATTGTGATAAGTGGACACGTACGCGATGTCATTGTGAAGGCAA
CAATTTTGGTATGCACATATAATTTTCACCTCTCGTTAATTTGGATATATATGTTAGAAA
ATCTCACAAACAAATAATTTATGAACTAATGTAGTGTTTATATGAGAAATATATATGACT
TAATTCAATCATGGTTTGAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA
TNTCGACGTGTCTACATTCCTCCCCAAA

Last Updated on 06.12.2018 16:11