Sequences from confirmation amplicon


Confirmation sequences for line 377D04 and hit At4g13340 are shown.
Line ID 377D04
Locus At4g13340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7758908
Primers used for PCR TM27 - 8474, show primer details
   
Sequence Name 56-K103693-0022-377-D04C-TM27
GenBank Accession LN509078 [GenBank]
Sequencing primer gene specific primer, TM27: 5'-CTTCCTCATATCTCCATATCCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K103693-0022-377-D04C-TM27
ATACAATCTACATTTCCCATTTTACCCTCGCTTCTCCTTCCAATTCTCACCCCATTGCCA
CTTCACTCAGATGTCTTGTACACTGACATGAAGAAGACGATTCAAATCCTCCTCTTCTTC
TTCTTCCTCATCAATCTCACCAACGCTCTCTCAATCTCCTCTGACGGCGGCGTTCTCTCC
GATAACGAAGTCCGTCACATTCAACGCCGTCAATTACTCGAATTCGCCGAACGAAGCGTC
AAAATCACCGTTGATCCTTCTCTAAACTTCGAGAATCCGAGATTGCGAAATGCTTATATA
GCTCTACAAGCTTGGAAACAAGCGATTCTCTCTGATCCAAACAATTTCACTTCGAATTGG
ATCGGATCCAATGTCTGTAACTACACAGGATATATTCAATTGTAAATGGCTTCATGTCCG
GGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAA
TTACCAATTTTTTTTCAATTCAAAAATGTAGA
   
Sequence Name 72-K103694-0022-377-D04C-8409
GenBank Accession LN512712 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K103694-0022-377-D04C-8409
GAGCCTTTACATTGAATATATCCTGTGTAGTTACGACATTGGATCCGATCCAATTCGAAG
TGAAATTGTTTGGATCAGAGAGAATCGCTTGTTTCCAAGCTTGTAGAGCTATATAAGCAT
TTCGCAATCTCGGATTCTCGAAGTTTAGAGAAGGATCAACGGTGATTTTGACGCTTCGTT
CGGCGAATTCGAGTAATTGACGGCGTTGAATGTGACGGACTTCGTTATCGGAGAGAACGC
CGCCGTCAGAGGAGATTGAGAGAGCGTTGGTGAGATTGATGAGGAAGAAGAAGAAGAGGA
GGATTTGAATCGTCTTCTTCATGTCAGTGTACAAGACATCTGAGTGAAGTGGCAATGGGG
TGAGAATTGGAAGGAGAAGCGAGGGTAAAATGGGAAATGTAGATTGTATAATAATACAAT
GTTATAAGAGTATTTTTGGGATATGGA

Last Updated on 06.12.2018 16:11