Sequences from confirmation amplicon


Confirmation sequences for line 377E05 and hit At1g79610 are shown.
Line ID 377E05
Locus At1g79610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29956088
Primers used for PCR 06ht - 8474, show primer details
   
Sequence Name 36-K112586-0022-377-E05C-06ht
GenBank Accession LN502283 [GenBank]
Sequencing primer gene specific primer, 06ht: 5'-CAATGAATCTCAACTAGGCAACAA-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K112586-0022-377-E05C-06ht
TTACAACCTGTAAGTGCAGAGACCCTCATGTCTTTATTGCTTTTCTTGTTTTACCATCAA
AGATCGCATCCTCGCTTTTAAGTTCTTGATGATGCTTACGTTGTTGTTGGGTTTTATGCA
GAAACCTTTCTTCTCAAACTTTGGAGCCATTGTCACTTTTTCAGTGCTTGGAACTTTTGT
AGCTTCCATGGTTACTGGATTGCTTGTGTAAGTATCTCTTTACTGTGAATGGCTAATGTT
TGTTGACGGAAAAGGTTCATGAACACGTACCCGGCCGTTCATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACAGTTATT
   
Sequence Name 10-K112585-0022-377-E05C-8409
GenBank Accession LN490728 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K112585-0022-377-E05C-8409
ATGAACGGCCGGGTACGTGTTCATGAACCTTTTCCGTCAACAAACATTAGCCATTCACAG
TAAAGAGATACTTACACAAGCAATCCAGTAACCATGGAAGCTACAAAAGTTCCAAGCACT
GAAAAAGTGACAATGGCTCCAAAGTTTGAGAAGAAAGGTTTCTGCATAAAACCCAACAAC
AACGTAAGCATCATCAAGAACTTAAAAGCGAGGATGCGATCTTTGATGGTAAAACAAGAA
AAGCAATAAAGACATGAGGGTCTCTGCACTTACAGGTTGTAAACTGAAGCCTGACTGGGT
GCAAACAAGTACGTCAAGGAAAACAACATATTTTGTTGCCTAGTTGAGATTCATTGA

Last Updated on 06.12.2018 16:11