Sequences from confirmation amplicon


Confirmation sequences for line 377H02 and hit At2g35585 are shown.
Line ID 377H02
Locus At2g35585 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:14937581
Primers used for PCR GS01 - 8474, show primer details
   
Sequence Name 15-K027062-022-377-H02C-8409
GenBank Accession LN492819 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K027062-022-377-H02C-8409
GTAGATTTCCCGGACATGTAGTTTTCTACAAAAAAAACTAATTTTTTCTTTTCTTGGAAC
TTTTTCAAGAATCTTACATTGAGAATGGATGGCTCTGGGAGTGGACATTGGATCGGTTGA
TCGTTGTCTAAGGGCTCCATCGGGTGATCCACCGGTTTAAACTCTGTTGCTACTTCGATC
CCATGAGTTTCAGTGGTTGTGGTATTAGTTGCCGGAGCAAGATCAGATCTTGGAGACAAT
ACTTCCCTGCCATCCTGATCATAGGAGAAGATTTCATGTCAAGTGTAGCCTGAAACCAAT
TTAGGGCTTTTCCAGATAAATAAAAGGGTCTCAGATTCAACTATAGCTTGAAATCTATTA
AAGGTAATCAGATTACAAAAGAACAGAGAGAATCTTGAACCAATGAAAGGAACTCAAAAG
AACAGAAGAAGCACTAAACATCTCCCATTCTAATCATCAACTCTTTGAGCCTAGTTAGCA
TCTATGATATGAGCAAAGAATCTGTCCAACAATTAAACAAATGTACTGGAGAAGTGTCAT
ACTAAAAAGGAATCACTATGAACATAAAGCTCAAGC
   
Sequence Name 16-K027062-022-377-H02C-GS01
GenBank Accession LN493286 [GenBank]
Sequencing primer gene specific primer, GS01: 5'-TCTGTTAGTTGTGTGCTTGAGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K027062-022-377-H02C-GS01
TATGTTCATAGNTGACTTCCTTTTTAGTATGACACTTCTCCAGTACATTTGTTTAATTGT
TGGACAGATTCTTTGCTCATATCATAGATGCTAACTAGGCTCAAAGAGTTGATGATTAGA
ATGGGAGATGTTTAGTGCTTCTTCTGTTCTTTTGAGTTCCTTTCATTGGTTCAAGATTCT
CTCTGTTCTTTTGTAATCTGATTACCTTTAATAGATTTCAAGCTATAGTTGAATCTGAGA
CCCTTTTATTTATCTGGAAAAGCCCTAAATTGGTTTCAGGCTACACTTGACATGAAATCT
TCTCCTATGATCAGGATGGCAGGGAAGTATTGTCTCCAAGATCTGATCTTGCTCCGGCAA
CTAATACCACAACCACTGAAACTCATGGGATCGAAGTAGCAACAGAGTTTAAACCGGTGG
ATCACCCGATGGAGCCCTTAGACAACGATCAACCGATCCAATGTCCACTCCCAGAGCCAT
CCATTCTCAATGTAAGATTCTTGAAAAAGTTCCAAGAAAAGAAAAAATTAGTTTTTTTTG
TAGAAAACTACATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATG
GAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGC
GTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA

Last Updated on 06.12.2018 16:11