Sequences from confirmation amplicon


Confirmation sequences for line 378A11 and hit At1g08930 are shown.
Line ID 378A11
Locus At1g08930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2874337
Primers used for PCR 02ip - 8474, show primer details
   
Sequence Name 36-K112877-0022-378-A11C-8409
GenBank Accession LN502290 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K112877-0022-378-A11C-8409
ATTTACATTGAATATATCACAGGCTGGCTTTGTGTAGCATTGGCTCAGGTTTTTTGTCTA
AACTACTTGTCGTTTGTCCTTTTGCTGACTTGTCACAATTTATTTATATTATCCTTTTAT
TAATTTCTGTGTTTTCTTAAAAAAATATTTTTATTTTAATATTTTGAAGAATGCAATGTG
GCTGGACTGTGGAAGATTGTTACTTGGAATCGGCGTTGGTATATTTAGCTACGTGGTAAT
TCCTCTTTTTCCAAATTCATTCTCATCACACAAATTTCTGTACTAAAATAAGAGAATTAC
TGATAAAAAGTTGTGTTTCATGTAGATTCCGGTGTATATAGCCGAAATTGCACCTAAACA
TGTCCGAGGATCGTTTGTGTTCGCCAATCAGGTACGTAAATTTCTATTTAATTTACTTTA
CTGTTATTTTTAAAACACATGTATTAAATTTTCGTTATATAACATGCTTTTTTTTTTGTT
AAAATGTGAACAGTTAATGCAAAATTGCGAAATTTCACTCTTCTTCATCATTGGCAATTT
TATTC
   
Sequence Name 73-K112878-0022-378-A11C-02ip
GenBank Accession LN512928 [GenBank]
Sequencing primer gene specific primer, 02ip: 5'-TAGTCTCCATGGAATAAAATTGCC-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K112878-0022-378-A11C-02ip
TCGCATTTTGCATCACTGTTCCATTTTAACAAAAAAAAAAGCATTTTATATAACGAAAAT
TTAATACATGGGTTTTTAAAAATAACAGTAAAGTAATTTAAATAAAATTTTACTTACCTG
ATTGGCAAACACAAACAATCCTCGAACATGTTTAGGTGCAATTTCGGCTATTTACCCCGA
ATTCTACTTGAAACACAACTTTTTATCAGTAATTCTCTTATTTTAGTACAAAAATTTGTG
TGATGAGAATGAATTTGGAAAAAAAGGAATTACCACGTACCTAATTATACCACCCCCAAT
CCCAAGTAACAATCTTCCACAGTCCACCCACTTTGCATTCTTCAAAATATTAAAATAAAA
ATATTTTTTTAAAAAAACCCAAAATTTAATAAAAGGATAATATAATTAATTGGTGACAAG
TCACCAAAAGGACAAACAACAAGTATTTTAAACAAAAAACCTGACCCAATGCTACACAAA
GCCGCCCGGTGATATATTCAATTGTAAATGGCTCCATGTCCGGGAAATCTACTTGGATCA
CCATTGAGTATAATGGTCAATATGGAGAAAAAAAAAAAGTATTTCCCATTTTTTTTTCAT
TTCAAAAATGTAAATTTCCGCACGGTTTT

Last Updated on 06.12.2018 16:11