Sequences from confirmation amplicon


Confirmation sequences for line 378D01 and hit At5g46600 are shown.
Line ID 378D01
Locus At5g46600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18910534
Primers used for PCR 50cp - 8474, show primer details
   
Sequence Name 62-K115963-0022-378-D01C-50cp
GenBank Accession LN510831 [GenBank]
Sequencing primer gene specific primer, 50cp: 5'-GCCAAAGATCCTGCTATTAGTGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K115963-0022-378-D01C-50cp
TGCACCTGAATTATATATACCCAACCAAGTTTTATGAAAATACCACTAATGAATTAAGCC
ACTAACAATTGAGGACCATGGCTAGATATTTTTCAAGATGCAAGTAACTAATCAATAAGC
CACTTAGTCTCAAAGAAATACGCCAAATGTTTTTGTCCTTCTGCCGTTCCAATTCCGCTT
TATTAATTGTGTATTAATTAAGCAAAAAAATTTCCATCAGAATCATAAAATATGGTACCA
TCTGAATTCGTAATTTCGTTTTTTCATATAACATAGCCGGTCCTGGACTGGAGTTTCAAA
CTCTAGAAGTGATAGTACCATTTATGGTGTAACGTCACTAGAGGAAACTTTTACTTTAAC
ATTGCTGAAATTAAATGTCATTATTAACGGCTGTAAGCCATTTGGCCTGCTTGGCCAGCT
CTGGATAAGATCACAACTCACAAGCAAAGAATGTAAATTATTGATTTAAGATCGTGTGAT
TGGAACAATAGATCCATGATTCCAATCACAAGATCTTTTAATACGAACAAGAGATCTTTC
TTGATCAAATTCTATGTCCTTGTAGAAAGATAATTAAGATCACAAGCAAAAAATGGTTAA
TATACTTACCGGCAGAGAACTCAAGAACCACAACGACGGTCATGACTGCCCAAAGAGCAT
TTTTTCCAACACCTTCGAAAAAAGGTTCCAGGATATATTCAATTGTAAATGGCTTCATGT
CCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAG
TAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 62-K115962-0022-378-D01C-8409
GenBank Accession LN510830 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K115962-0022-378-D01C-8409
ATTTCATTGAATATATCCTGGAACCTTTTTTCGAAGGTGTTGGAAAAAATGCTCTTTGGG
CAGTCATGACCGTCGTTGTGGTTCTTGAGTTCTCTGCCGGTAAGTATATTAACCATTTTT
TGCTTGTGATCTTAATTATCTTTCTACAAGGACATAGAATTTGATCAAGAAAGATCTCTT
GTTCGTATTAAAAGATCTTGTGATTGGAATCATGGATCTATTGTTCCAATCACACGATCT
TAAATCAATAATTTACATTCTTTGCTTGTGAGTTGTGATCTTATCCAGAGCTGGCCAAGC
AGGCCAAATGGCTTACAGCCGTTAATAATGACATTTAATTTCAGCAATGTTAAAGTAAAA
GTTTCCTCTAGTGACGTTACACCATAAATGGTACTATCACTTCTAGAGTTTGAAACTCCA
GTCCAGGACCGGCTATGTTATATGAAAAAACGAAATTACGAATTCAGATGGTACCATATT
TTATGATTCTGATGGAAATTTTTTTGCTTAATTAATACACAATTAATAAAGCGGAATTGG
AACGGCAGAAGGACAAAAACATTTGGCGTATTTCTTTGAGACTAAGTGGCTTATTGATTA
GTTACTTGCATCTTGAAAAATATCTAGCCATGGTCCTCAATTGTTAGTGGCTTAATTCAT
TAGTGGTATTTTCATAAAACTTGGTTGGGTATATATAATTTCAGGTGCAACATTACGCAA
GGGATTAAATAGAGGATTAGGGACACTAATAGCA

Last Updated on 06.12.2018 16:11