Sequences from confirmation amplicon


Confirmation sequences for line 378E04 and hit At1g13080 are shown.
Line ID 378E04
Locus At1g13080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4460046
Primers used for PCR 27sa - 8474, show primer details
   
Sequence Name 55-K116229-0022-378-E04C-27sa
GenBank Accession LN508912 [GenBank]
Sequencing primer gene specific primer, 27sa: 5'-TGTGGTTCAAATACTCAACTTTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K116229-0022-378-E04C-27sa
CCTTTCTTCATTCACTTCTAGGGGTTTTCTAATAAGTTTCGTTCATCCGCCCAAATCATT
GTTACCGCGCTTGTATCTATCCCCGCGAGAAATACATCCTAAATACCACATAATAATCGA
TACAAAATTTTTTGAATCAATATAGGTTAACATAGGATATAAAGAAAACCAAAATTTGAG
AATTTACCATGACAATTCGCCTTGAGATTATCCATGTTGAGTTTGAAAGAATCTGAATCT
TCTTGTTTATCGATCATATCCAAAATCAAGGTGACAATATCTTGATTCTTTCTTCCCTCT
GGCAATTCAATTGAATATATGCTGGTGCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 15-K116228-0022-378-E04C-8409
GenBank Accession LN493130 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K116228-0022-378-E04C-8409
GATAAACAAGAAGATTTCAGATTTCTTTCAAACTCAACATGGATAATCTCAAGGCAATTG
TCATGGTAAATTTCTCAAATTTTGGTTTTTTTTATATCCTATGTTAACCTATATTGATTC
AAAAATTTTGTATCATTATTATGTGGTATTTAGGATGTATTTCTCGCGGGGATAGATACA
AGCGCGGTAACAATGATTTGGGCGATGACAGAACTTATTAGAAACCCTAGAGTGATGAAG
AAGGCTCAAGAAAGTATTCGAACCACTCTGGGTCTCAAGAAGGAAAGAATCACTGAAGAA
GATTTAGGCAAAGTTG

Last Updated on 06.12.2018 16:11