Sequences from confirmation amplicon


Confirmation sequences for line 378G11 and hit At2g41370 are shown.
Line ID 378G11
Locus At2g41370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17239267
Primers used for PCR BN08 - 8474, show primer details
   
Sequence Name 68-K115963-0022-378-G11C-BN08
GenBank Accession LN512080 [GenBank]
Sequencing primer gene specific primer, BN08: 5'-CTCCCTCTGCTTTCACTCTTCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K115963-0022-378-G11C-BN08
ACTTTTTTGTTCTTGTTCTGCTTCTTTTAGAGTGTTTTCTTTCATGTGCTTGGCTTAATT
TTGGTAAATTACGAAACGCTTTTGATTCGATTTTTATTTGTCTTTCTTTGGGTTTGGGTT
AAATTTATTACAAATCGCGAAACCCTTTTGGTCTGATTATTTTCTTTCATGGGGTATTTC
GCTTAAGATTTTTAATTTTTAATAACAATTTTTTATTTGTTAAATTATATATGTTCTTGT
CTTGATTTCATGTATAAATGAGTTTAAGTTTCTTGCTTTGGAACAATTTTCATCGGATAA
AGTTTCCGAAAGCAGACGAACGGTGGTTTTATTGCACGGAAGAGAATTTTCCTAAAAGTA
GAAATCTATGTTATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGT
ATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAAT
GTAGATGTCCG
   
Sequence Name 68-K115962-0022-378-G11C-8409
GenBank Accession LN512079 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K115962-0022-378-G11C-8409
GCATTACATAACATAGATTTCTACTTTTAGGAAAATTCTCTTCCGTGCAATAAAACCACC
GTTCGTCTGCTTTCGGAAACTTTATCCGATGAAAATTGTTCCAAAGCAAGAAACTTAAAC
TCATTTATACATGAAATCAAGACAAGAACATATATAATTTAACAAATAAAAAATTGTTAT
TAAAAATTAAAAATCTTAAGCGAAATACCCCATGAAAGAAAATAATCAGACCAAAAGGGT
TTCGCGATTTGTAATAAATTTAACCCAAACCCAAAGAAAGACAAATAAAAATCGAATCAA
AAGCGTTTCGTAATTTACCAAAATTAAGCCAAGCACATGAAAGAAAACACTCTAAAAGAA
GCAGAACAAGAACAAAAAAGTGGAAACTTTCAATTTTGTTTTCTTGTGATGTGCATGGAG
TAAGAAGAGTGAAACAGGAGGGAG

Last Updated on 06.12.2018 16:11