Sequences from confirmation amplicon


Confirmation sequences for line 378H10 and hit At5g11950 are shown.
Line ID 378H10
Locus At5g11950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3855563
Primers used for PCR 07ay - 8474, show primer details
   
Sequence Name 65-K118471-0022-378-H10C-07ay
GenBank Accession LN511495 [GenBank]
Sequencing primer gene specific primer, 07ay: 5'-TAAGATTAGGGGCACGATACAAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>65-K118471-0022-378-H10C-07ay
ATCTTAAATCATACAAACGCAGGCAAGAACCAAGAGCTAGTAATGCAAAAAATGAATAAC
TCGCAGACACATGATTCATCATTAGACAAACTTCAAAACTCCAGCCAAGTTTACTACTTC
CTGGACAAATGAGCTTCTAGAAGAAGATACATACCTCAATTGGCATCAAAGCTTTGGGAA
TGATTCTGCAATCACAGAGAATCCAATCACTCTAAGTTTGAAAATAAAAGAGATTGAAAG
AGCTATAAAACTAATTGAGGATTCTTAAACTAACCCAAGTACATGTAAACCACCTTCATA
GACTCTCCTGGATATCAAACCCATCAATCCAACACTTCCTCCTCCATAAACCAAATCTAT
CTTCCTCTTCACCTACACATTCCAAAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 33-K118470-0022-378-H10C-8409
GenBank Accession LN501187 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K118470-0022-378-H10C-8409
GGAGGAAGTGTTGGATTGATGGGTTTGATATCCAGGAGAGTCTATGAAGGTGGTTTACAT
GTACTTGGGTTAGTTTAAGAATCCTCAATTAGTTTTATAGCTCTTTCAATCTCTTTTATT
TTCAAACTTAGAGTGATTGGATTCTCTGTGATTGCAGAATCATTCCCAAAGCTTTGATGC
CAATTGAGGTATGTATCTTCTTCTAGAAGCTCATTTGTCCAGGAAGTAGTAAACTTGGCT
GGAGTTTTGAAGTTTGTCTAATGATGAATCATGTGTCTGCGAGTTATTCATTTTTTGCAT
TACTAGCTCTTGGTTCTTGCCTGCGTTTGTATGATTTAAGATCAGGAGAGAAAACAATGT
TCTTTGGGGATTGTATGGTTTGTATCGTGCCCCTAATCTA

Last Updated on 06.12.2018 16:11