Sequences from confirmation amplicon


Confirmation sequences for line 379C05 and hit At2g21940 are shown.
Line ID 379C05
Locus At2g21940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9351262
Primers used for PCR 19ve - 8474, show primer details
   
Sequence Name 70-K118051-0022-379-C05C-19ve
GenBank Accession LN512461 [GenBank]
Sequencing primer gene specific primer, 19ve: 5'-TAAAAATCCTTTCCCTTTTACGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K118051-0022-379-C05C-19ve
CGAATTTTTTTCGTTTGGAAGCTTGGAGAAAAATTATACTCTTCTCATTGAATGATCAAT
TTACAAATCCTCATTTGATCGATCTTGTCAATTTTTGAAAAAAAAAATCTGAACTTTGAG
AAAAGTTATTGAGTTTCGATATCTGTCGGTCATGGTTTGATTGTGGCTGTTGGGTAAAAC
AACAAATTAGGTAGGGTTTTGTGATATTGATGTCCGTAGTCCTTAATTATGGCCAAATTG
TTGTCTTTTAGGTTTTGACAGTAGCAAAGAAAAGCTGAATCTAGTTAGAAATTGCTTTTG
CAGGGTTTTTGAATAGTTTGTGTATTGAAAATTGTCTGAAATGGAAGCAGCTATTACTCA
AAGGATTCAGTACCCATCAGGGGTTGATTGTAAAAAAGTTGAGTGTAACCCGCACCGTGG
TTCATTGCGGTATTCTCAGCAGGTTAAGGTAGATAGAAGGTTTAGAGGTCTTTCTTTGGC
TCGCTTGCAACCTGAAAATGAGTATGATCATGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAAAATCC
   
Sequence Name 34-K118050-0022-379-C05C-8409
GenBank Accession LN501586 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K118050-0022-379-C05C-8409
GATCATACTCATTTTCAGGTTGCAAGCGAGCCAAAGAAAGACCTCTAAACCTTCTATCTA
CCTTAACCTGCTGAGAATACCGCAATGAACCACGCTGCGGCTTACACTCAACTTTTCTAC
AATCAACCCATGATGGGTACTGAATCCTCTGAGTAATAGCTGCTTCCATTTCAGACAATC
TTCAATACACAAACTATTCAAAAACCCTGCAAAAGCAATTTCTAACTAGATTCAGCTTTT
CTTTGCTACTGTCAAAACCTAAAAGACAACAATTTGGCCATAATTAAGGACTACGGACAT
CAATATCACAAAACCCTACCTAATTTGTTGTCTTACCCAACAGCCACAATCAAACCATGA
CCGACAGATATCGAAACTCAATAACTTTTCTCAAAGTTCAGATTTTTTTTTTCAAAAATT
GACAAGATCGATCAAATGAGGATTTGTAAATTGATCATTCAATGAGAAGAGTATAATATT
TTCTCCAAGCTTCCAAACAAAAAAAAATTCAAACTTTCCAAAGAAAAAGAGAATTATTAT
CCAAATCAAATTGAACCGTAAAAGG

Last Updated on 06.12.2018 16:11