Sequences from confirmation amplicon


Confirmation sequences for line 379F03 and hit At2g19360 are shown.
Line ID 379F03
Locus At2g19360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8379305
Primers used for PCR BW77 - 8474, show primer details
   
Sequence Name 24-K106020-0022-379-F03C-BW77
GenBank Accession LN496997 [GenBank]
Sequencing primer gene specific primer, BW77: 5'-ACACATTTATAAGCGTTTGGGTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K106020-0022-379-F03C-BW77
ATTTTCTCCCTTTAGTATGGCATACTGTATATTAAGTATGTAAATATGTAAGAAACTATA
AAAACATATATACTCATAAAAGGAAAAATGATGAATGATATATGCACCTCATGTGTACCG
TCGTTATTAGTTGGATTCGTTGGTTTATGTTGATGGATATTGTTGGGATCTTTTTTCCCA
AACCTTTCAATTGATTTTGCTCGTAAAATATCTTCTTTTCTCGTTCTTCTGATGGGAATA
CTATTTTTTGGACATTTTCCATTGATTGTCCAAAGTTGATCAGCAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATTA
   
Sequence Name 24-K106021-0022-379-F03C-8409
GenBank Accession LN496998 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K106021-0022-379-F03C-8409
CTTTGGACATCATGGAAAATGTCCAAAAATAGTATTCCCATCAGAAGAACGAGAAAAGAA
GATATTTTACGAGCAAAATCAATTGAAAGGTTTGGGAAAAAAGATCCCAACAATATCCAT
CAACATAAACCAACGAATCCAACTAATAACGACGGTACACATGAGGTGCATATATCATTC
ATCATTTTTCCTTTTATGAGTATATATGTTTTTATAGTTTCTTACATATTTACATACTTA
ATATACAGTATGCCATACTTAAAGTGGGAGAAAATTCTCCTCGAGCTAAATTTTATGGAA
CCCAAACGCTTATAAATGTG

Last Updated on 06.12.2018 16:11