Sequences from confirmation amplicon


Confirmation sequences for line 379H03 and hit At3g29763 are shown.
Line ID 379H03
Locus At3g29763 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:11594913
Primers used for PCR 18xh - 8474, show primer details
   
Sequence Name 59-K119070-0022-379-H03C-18xh
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 18xh: 5'-TGTGAAGAATGATTATCGAATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K119070-0022-379-H03C-18xh
GGATTACCATTTCGTGGTCATGATGAATCAGAAGAGTCAACAAATAAGGGTAATTTTTTA
GAGCTTTTGAAGTATATAGCTGGTCAGAATGAAGTTGTAAAGAAAGTTGTGTTGAAAAAT
GCTCCTAAAAATAACCAGATGACATCTCCACCAATTCAAAAAGATATTGTCCATTGCTTT
TCAGAAGAGGTAACTAGATCTATTATTGAAGAAATGGATAATGATGTCTTTGGCTTGCTT
GTAGATGAATCGGCTGATGCTTCAGATAAAGAGCAAATGGCAGTAGTTTTTCGCTTCGTT
GATAAGTATGGGGTAGTTAAAGAAAGATTTATTGGTGTTATTCATGTGCAAGAGACATCT
TCTTCATTCAGGATATATTCAGGATATATTCGGTGTTATTCAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCATT
ATT
   
Sequence Name 27-K119069-0022-379-H03C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K119069-0022-379-H03C-8409
ATTGAATATATCCTGAATAACACCGAATATATCCTGAATATATCCTGAATGAAGAAGATG
TCTCTTGCACATGAATAACACCAATAAATCTTTCTTTAACTACCCCATACTTATCAACGA
AGCGAAAAACTACTGCCATTTGCTCTTTATCTGAAGCATCAGCCGATTCATCTACAAGCA
AGCCAAAGACATCATTATCCATTTCTTCAATAATAGATCTAGTTACCTCTTCTGAAAAGC
AATGGACAATATCTTTTTGAATTGGTGGAGATGTCATCTGGTTATTTTTAGGAGCATTTT
TCAACACAACTTTCTTTACAACTTCATTCTGACCAGCTATATACTTCAAAAGCTCTAAAA
AATTACCCTTATTTGTTGACTCTTCTGATTCATCATGACCACGAAATGGTAATCCTTGAT
GTAACAAGTGTCGAGAAACATCAATGGAAGCATTCAACCGGATTCGATAATCATTCTTCA
CA

Last Updated on 06.12.2018 16:11