Sequences from confirmation amplicon


Confirmation sequences for line 379H09 and hit At5g58940 are shown.
Line ID 379H09
Locus At5g58940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23799517
Primers used for PCR JY13 - 8474, show primer details
   
Sequence Name 01-K038035-022-379-H09C-JY13
GenBank Accession LN486489 [GenBank]
Sequencing primer gene specific primer, JY13: 5'-TCTTGAATATGTGCAGTATCAGGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K038035-022-379-H09C-JY13
CAGCTCCTTTGGAGGTCAACAGAAAGAAAGGTTTCAGGTCAATATCGTTTTTCTGGTTCA
AGATTTCAAAGTCCTGGGAAAGATTCTTCTTCTAGCAAAAGTTGGCATCAAGGCCCGGTA
ATATTTTCTTTTGGGGAGCTTCAAAGGGCAACCGCAAATTTTTCTTCGGTTCATCAAATT
GGGGAAGGCGGTTTTGGAACTGTTTTTAAGGGAAAACTTGATGATGGAACTATTGTTGCT
ATCAAGCGTGCGAGAAAGGTAGTAGTTGAAATCCTTTTCTTGGAAAATATGTGGAAAACA
GATTGAACTAGTTTCTTAAGAATTATTCGGAGAAGACATTATCGATAACTCAAAAGATGG
ATCATGTCCATAAAGACATATATCCATCATATTCAATTGTAAATGGCTTCATGTCCGGGA
AATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTA
CCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTAC
ATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAAT
TATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTCAC
   
Sequence Name 41-K038035-022-379-H09C-8409
GenBank Accession LN503998 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K038035-022-379-H09C-8409
TCCGGACTCAGCCATTTCAATTGAATATGATGGATATATGTCTTTATGGACATGATCCAT
CTTTTGAGTTATCGATAATGTCTTCTCCGAATAATTCTTAAGAAACTAGTTCAATCTGTT
TTCCACATATTTTCCAAGAAAAGGATTTCAACTACTACCTTTCTCGCACGCTTGATAGCA
ACAATAGTTCCATCATCAAGTTTTCCCTTAAAAACAGTTCCAAAACCGCCTTCCCCAATT
TGATGAACCGAAGAAAAATTTGCGGTTGCCCTTTGAAGCTCCCCAAAAGAAAATATTACC
GGGCCTTGATGCCAACTTTTGCTAGAAGAAGAATCTTTCCCAGGACTTTGAAATCTTGAA
CCAGAAAAACGATATTGACCTGAAACCTTTCTTTCTGTTGACCTTCCAAAGGAGCTTGGT
GATGAATAACCAGTCCAGCCTGATACTGCCATAATTCAAGA

Last Updated on 06.12.2018 16:11