Sequences from confirmation amplicon


Confirmation sequences for line 380B06 and hit At3g59170 are shown.
Line ID 380B06
Locus At3g59170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21878837
Primers used for PCR BW79 - 8474, show primer details
   
Sequence Name 25-K106020-0022-380-B06C-BW79
GenBank Accession LN497449 [GenBank]
Sequencing primer gene specific primer, BW79: 5'-GCGTTATCTACTTGCTTTTGTTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K106020-0022-380-B06C-BW79
CTGCATCGCGATAAAGGGTAAAGAATACTTTACATGAGAAAGGTTTTGTTGGTTTCGTGC
TTTTGGTTAACAATAAAAGGAAGAAACTTTCGACAAGCTTTCCTGATTTCGTAGATAGGA
TCCTTGCTTTGCAAGGCAATTCTCCTCTAGACAAATTCTCTCTAAAGATGGTTGATGGCC
ATGATCCTGTTGATCCAGATAGTGTCGTTCCTTGGATACACAAGGTGTTGGTACGTGGTG
TATCGGATCTCCATCTGGTCGTAGACATGAATGAATGGACATCTGATCCTCTTCCTTCAA
GGATCTTCTTGAGCGAGACATTGGTTAAGCTAACTATCAAAATTAGAGATGGCCCTTTCA
TTGATGTGAAACATGTTCATCTTCCAAAGCTTAAGACTCTATACCTCCAGTCAGTTATGT
TTGATGAGAACGATATTGGGTTTAGAAAGCTCCTTTCTGGTTGTCCCGTGCTCGAAGAAC
TTGTTCTCGATGGTATGGGGTCAATATGGCTTCATGTCCGGGAAATCTACATGGATCAGC
AATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATT
CAAAAATGTAGATGTCCGCACGGNTTATTA
   
Sequence Name 25-K106021-0022-380-B06C-8409
GenBank Accession LN497450 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K106021-0022-380-B06C-8409
ATTGACCCCATACCATCGAGAACAGTTCTTCGAGCACGGGACAACCTGAAAGGAGCTTTC
TAAACCCAATATCGTTCTCATCAAACATAACTGACTGGAGGTATAGAGTCTTAAGCTTTG
GAAGATGAACATGTTTCACATCAATGAAAGGGCCATCTCTAATTTTGATAGTTAGCTTAA
CCAATGTCTCGCTCAAGAAGATCCTTGAAGGAAGAGGATCAGATGTCCATTCATTCATGT
CTACGACCAGATGGAGATCCGATACACCACGTACCAACACCTTGTGTATCCAAGGAACGA
CACTATCTGGATCAACAGGATCATGGCCATCAACCATCTTTAGAGAGAATTTGTCTAGAG
GAGAATTGCCTTGCAAAGCAAGGATCCTATCTACGAAATCAGGAAAGCTTGTCGAAAGTT
TCTTCCTTTTATTGTTAACCAAAAGCACGAAACCAACAAAACCTTTCTCATGTAAAGTAT
TCTTTACCCTTTTATCGCGATGCAGATAGACAGAGTCATCAAATTCAAGGTTTGGAACAA
AAGCAGTAGAATAACG

Last Updated on 06.12.2018 16:11