Sequences from confirmation amplicon


Confirmation sequences for line 380B09 and hit At1g27000 are shown.
Line ID 380B09
Locus At1g27000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:9374038
Primers used for PCR KP75 - 8474, show primer details
   
Sequence Name 10-K034064-022-380-B09C-8409
GenBank Accession LN490555 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K034064-022-380-B09C-8409
AAGCCATTTACAATTGAATATATCCTGGTCGGCGGCGGTGTAAAAATAGTCGGAGCTATG
GCGATGCAGGCCGGAGTTGGCTTATCCAGGATCTTTCTCTTAGCCGGAGCAGGTACTTCA
CTTTGATTTCTCTGCTCAATCTAACTCGCTCGTTTACTTACTGCTAATTGGGATCTGTAT
ATCCTCCTGATACGTTTTTCGATTTGATGGAATCAAGGTTACACTGGTACGATCATGATG
AAGAATGGAAAATTGTCGGATTTGTTGGGTGAATTGCAGGTACTTTTTTGATTCTATGCA
TTTTTTTTTTTGTTTCTCTCCTTGATTTTTTTATTTTTGACTAATTGATTTGTGCTTTCA
ATAAAGTTTGGTGAAGGGAATGGAAAAATCTGGAAAAGGGTCTAAGGGAAATTCTGATGT
TTCTAATGCTATAGCTGCACAGGTACATAAACTCATATTCATTGGATATAGGAATATTGG
TGAGGGATGGTATTGATATCTTTTGCAATATTGGTGAAAAGGTTCCCCGGTTGGCTATGG
AAATTCAACAGCTAGCTTCCCACCAACACATAACGGTCATGAATGGAGTTTCTGGTGGTA
TGTGAAAATTAGGGATTTGTATTTCTACTCAAATAGTTTTGCTAAGTGTGTCTCTGTGTG
TGTGTGTGTTCATCAAACTCTCTTGTTTTTTCAGCAAACCTACAACCCCTTGCTGTTCCT
GCTGCGGCATTGGGAGCTCTCGAATATGGTTATATGTGGTGGAAGGTGGGATTACTAATT
TTTCAATCTCTTCTCTTG
   
Sequence Name 77-K034064-022-380-B09C-KP75
GenBank Accession LN513493 [GenBank]
Sequencing primer gene specific primer, KP75: 5'-TTTCAACAAAACAGCAGAATTCAC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K034064-022-380-B09C-KP75
CACATATAACNCATATCCGAGAGCTCCCAATGCCGCAGCAGGAACAGCAAGGGCTTGTAG
GTTTGCTGAAAAAACAAGAGAGTCTGATGAACACACACACACACAGAGACACACTTAGCA
AAACTATTTGAGTAGAAATACAAATCCCTAATCTTCACATACCACCAGAAACTCCATTCA
TGACCGTTATGTGTTGCTGCGAAGCTAGCTGTCGAATCTCCATAGCCAACCGGCGAACCT
TCTCACCAATATTGCAAAAGATATCAATACCATCCCTCACCAATATTCCTATATCCAATG
AATATGAGTTTATGTACCTGTGCAGCTATAGCATCAGAAACATCAGAATCTCCCTCAGAC
CCTTCTCCAGATTTCTCCATTCCCTTCACCAAACTCTATCGAAAGCACAAATCAATTAGT
CAAAAATAAAAAAATCAAGGAGAGAAACAAAAAAAAAAATGCATAAAATCAAAAAAGTAC
CTGCAATTCACCCAACAAATCCAACAATTTTCCATTCTTCATCATGATCGTACCAGTGTA
ACCTTGATTCCTTCAAATCGAAAAACGTATCAGGAGGATATACAGATCCCATTTAGCAGT
AAGTAAACGAGCGAGTTAAATTGAGCAAAAAAATCAAAGTGAAGTACCTGCTCCGGCTAA
AAAAAAAATCCGGGATAACCCAACTCCGGCCTGCATCGCCATAGCTCCAACTATTTTTAC
ACCGCCGCCAACGGGATATATTCAATTGTAAATGGCTTCATGTCCGGAAAATCTACATGG
ATCACCAATGAGTATGATGGTCAATATGGAA

Last Updated on 06.12.2018 16:11