Sequences from confirmation amplicon


Confirmation sequences for line 380B12 and hit At4g14305 are shown.
Line ID 380B12
Locus At4g14305 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:8235926
Primers used for PCR 52ra - 8474, show primer details
   
Sequence Name 39-K115806-0022-380-B12C-52ra
GenBank Accession LN503474 [GenBank]
Sequencing primer gene specific primer, 52ra: 5'-AATGTGATGATCTCTTTTGTGCAG-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K115806-0022-380-B12C-52ra
TTTGGTCCTTTTTCCCAATTGATGGATACCATTTTCAAAGGGAAGAAGGGTAATTCAACT
GTTGCCAAAAAGGTTTTGTCTTTTGAACCAAACTATCATCTTCTTGAATGCTTTTTGAAT
TCTTGGTTATATAACAAGTCAAACACTTGTTTTCCCAGTTTGTTTGTATCCATCCTTGGA
ATTGGATTCTAGATTACAACAACTTGTTTAACTTATATTCTGTTAGTTTCCTTGTTGCAA
TTTGGAATTTGAATGTGCTTAAATTAGTATAATCGTATGATCAATATATGTACAGAGATC
TCTTACTGTGTGCTTAACAAAAACTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 18-K115805-0022-380-B12C-8409
GenBank Accession LN494447 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K115805-0022-380-B12C-8409
CATTTACGTTTTTGTTAAGCACACAGTAAGAGATCTCTGTACATATATTGATCATACGAT
TATACTAATTTAAGCACATTCAAATTCCAAATTGCAACAAGGAAACTAACAGAATATAAG
TTAAACAAGTTGTTGTAATCTAGAATCCAATTCCAAGGATGGATACAAACAAACTGGGAA
AACAAGTGTTTGACTTGTTATATAACCAAGAATTCAAAAAGCATTCAAGAAGATGATAGT
TTGGTTCAAAAGACAAAACCTTTTTGGCAACAGTTGAATTACCCTTCTTCCCTTTGAAAA
TGGTATCCATCAATTTGTGGAAAAAGTGACCAAATGGTCCTCCATAAGCAAAGCCATACA
ACTGCACAAAAGAGACNATCACAT

Last Updated on 06.12.2018 16:11