Sequences from confirmation amplicon


Confirmation sequences for line 380C12 and hit At1g32780 are shown.
Line ID 380C12
Locus At1g32780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:11870413
Primers used for PCR 18st - 8474, show primer details
   
Sequence Name 48-K116569-0022-380-C12C-18st
GenBank Accession LN506616 [GenBank]
Sequencing primer gene specific primer, 18st: 5'-GCTCATTGGTAATAAAAGGTTCCA-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K116569-0022-380-C12C-18st
CATTATATCACTAAAACCAAAATTACTTCTTGTTTCTAAGATTAGAAATTAGAGACAAAT
TTCAGACCCCTTTCATGCATTGTTGGGCGAAATTTGGAAGCTGAGATTTGGGCTTGAAGC
CACCAAACACAGAACCGGTGATTCTACGGCCGTCGAAAAGCTCCATTGGGTGAAGAGGCA
ATGTTCTTGGCGTCGGATAGATCCCAACAAGTACTGTCGATCCCCAACCCTATCATCAAA
ATAATGTTTCTCATTTCCTTCTTTATCAAGTAACTAAATCAACATCAATTACGATCAGCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CACGATTATTAT
   
Sequence Name 19-K116568-0022-380-C12C-8409
GenBank Accession LN494921 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K116568-0022-380-C12C-8409
TTGCTGATCGTAATTGATGTTGATTTAGTTACTTGATAAAGAAGGAAATGAGAAACATTA
TTTTGATGATAGGGTTGGGGATCGACAGTACTTGTTGGGATCTATCCGACGCCAAGAACA
TTGCCTCTTCACCCAATGGAGCTTTTCGACGGCCGTAGAATCACCGGTTCTGTGTTTGGT
GGCTTCAAGCCCAAATCTCAGCTTCCAAATTTCGCCCAACAATGCATGAAAGGGGTCTGA
AATTTGTCTCTAATTTCTAATCTTAGAAACAAGAAGTAATTTTGTGTTTTAGTGATATTA
ATTGGAATCTTTGATTTGTAGGTTGTGAAATTGGAACCTTTTATANCCAATGAGC

Last Updated on 06.12.2018 16:11