Sequences from confirmation amplicon


Confirmation sequences for line 380E04 and hit At1g74180 are shown.
Line ID 380E04
Locus At1g74180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27899265
Primers used for PCR 08zh - 8474, show primer details
   
Sequence Name 20-K125762-0022-380-E04C-08zh
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 08zh: 5'-ATTGACAAATTAACCAAGGTACGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K125762-0022-380-E04C-08zh
TTGTTATGCATCCTCAATACGATCAATGAAGTTAGTCTCGTTTGTATTGGAAGAATAGGG
CCACTAAAACTGTTGTGAGAGAGCTGCAGGGTTATTAATGAAAAACAACCTGTTAATAAG
CTTCTAGGTAGCTCCCCAAAGAAATTATTGTAAGACAAGTCCAGGAATGAAATGTCATTC
ATCTCACCCATGGACGATGGAAGATTCCCTTGAAACCCATTATGAGATCCATTCATATGT
AGCAGCCTTGGAAGCACATGGCCAATATTATCAGGGAGCACTCCGGTGATATCATTTGCT
GAAAAATCCAAGACCTGCAACTTATGCACTATTGTAGGAATCTGAAAGATAGTGAAGGAA
TTATTCTTCAACTGTAAGACTTTAAGCTCTGGATTATTCTCCAACAGCCAGGTAGGAATG
TCTCCGGATAATCTATTGCTGGATAGATCAACTAGACGCAAATTTGTTTGGTAGACGAGA
AAATTAGGGATTTTCCCCAAGCTGCAAAAGGGTAGTGCAGCAACTGTCAGTTGAAATTTT
GGCAGCCAATTGCTCTCTGTCTCTACTTGTAACATCTCAGATGTTGACGAAAGTCTGAAC
ACCTTGAGCTTTGTGAGGTTGGCGAGTGGATTGAGTGAGAAGAAGCCTTCAAAGTTATTA
TCTGACAATGATAGATATTCGAGGGACTCGAGGCTATTGAAACTAGCTGGTAGATTTCCA
CTTAACTGGTTTGATGAGAGATCAAGAACCCGCAGCTTGTTCAAGTTACCTAAACACACA
GGAAGCTGACCTTCGAAATAATTTCCACGGAGATCAAGCTGACGCANATTCTTCATGTAG
AAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
   
Sequence Name 08-K125761-0022-380-E04C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K125761-0022-380-E04C-8409
TTCTTGATCTCTCATCTAACCGGAATAAGTGGAAATCTACCAGCTAGTTTCCATAGCCCC
CAGTCCCTCGTATATGTATCATTGTCAGATTATAAGTTTGAAGGCTTCTTCTCACTCAAT
CCACTCGCCAAACTCACAAAGCGTAAGGAGTTCAGACTTTCCTCAACATCTGAGATGTTA
CAAGTAGAGACAGATAGCTGTTGGCTGCCAAAATTTCAACTGACGGTTGCTGCACTACCC
TTTTGGGGCTAGGGGAAAATTTTTAATTTTCTCGTCTACAAAACAAATTTGCGTCTAGTT
GATCTATCCAGCAATACATTATCCGGACACATTCGTACCTGGATGATGGAGAATAATCCA
GAGCTTAAAGTCTTACAGATGAAGAATAATTCCTTCACTATATTTCAAATTCCTACAATA
ATGCATAAGTTGCGGGTCTTGGATTTTTCAGCAAATGATATCAGCGGACTGCTCCCTGAT
AATATTGGCCTTGTGCGTCCCAGGCTGCTACATGTGAATGGATCTGGTATTGGGGGTCAA
GGGAATCTTCCATCGGCGATGGGAGAGATGAATGACATTTCGATCCTGGACTTGACTAAC
AATCAGGGCACGGGGGAGCTACCTAGAAGCTTAGTAGCATGTTGTTTTTCATTAATAACC
C

Last Updated on 06.12.2018 16:11