Sequences from confirmation amplicon


Confirmation sequences for line 380E11 and hit At5g42570 are shown.
Line ID 380E11
Locus At5g42570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:17022114
Primers used for PCR 26lm - 8474, show primer details
   
Sequence Name 52-K113947-0022-380-E11C-26lm
GenBank Accession LN507885 [GenBank]
Sequencing primer gene specific primer, 26lm: 5'-AACAATGATCCACCTCCTTTACAC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K113947-0022-380-E11C-26lm
TCTTCTCTCCTCTCAAACTCCTCTCCGAAGCTAATCATCCTCACCTTCGATCGAATCAAA
CGTGGCCGTGGTCCCGTCGTCGTCAAAACCATAGGAACCACCGTATTCGTCGTTCTCTTG
TCGAGTATCTACAGTTTGGTTAATATCCAACGCCGATCTGAAGATGGTGCTGTTCTTAAT
CCCACTGATCAAGTCCTTGCGTCTAAGCATCTTCTCGAAGCTTCTCTTATGGGTATAAAT
CACAAAATCCTCGATCTCTTAAATCCTTGATCATAAGATCTGATATGAATTTGATTTGCT
TTTTGAAGAATTGTGATGTTTCTCTTAAATCAGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGATTATTA
   
Sequence Name 24-K113946-0022-380-E11C-8409
GenBank Accession LN497077 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K113946-0022-380-E11C-8409
ATTTCATTGAATTATCCTGATTTAGAGAAACTCACAATTCTTCAAAAAGCAAATCAAATT
CATATCAGATCTTATGATCAAGGATTTAAGAGATCGAGGATTTTGTGATTTATACCCATA
AGAGAAGCTTCGAGAAGATGCTTAGACGCAAGGACTTGATCAGTGGGATTAAGAACAGCA
CCATCTTCAGATCGGCGTTGGATATTAACCAAACTGTAGATACTCGACAAGAGAACGACG
AATACGGTGGTTCCTATGGTTTTGACGACGACGGGACCACGGCCACGTTTGATTCGATCG
AAGGTGAGGATGATTAGCTTTCGGAGAGGAGTTTTGAAGAGGAGGAGAAGGATCAGTGCC
ATTTCGGCGAAGATCACTGTGTAAAGGAGGTGGNTCATTGT

Last Updated on 06.12.2018 16:11