Sequences from confirmation amplicon


Confirmation sequences for line 380F11 and hit At4g21450 are shown.
Line ID 380F11
Locus At4g21450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:11426385
Primers used for PCR GS05 - 8474, show primer details
   
Sequence Name 17-K027064-022-380-F11C-8409
GenBank Accession LN493709 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K027064-022-380-F11C-8409
GTAGATTTCCCGGACATGAAGCCAAAATCAACATCCGACTCCTTCTCCTTCAGTTTCATC
TGTTGCTAAGTCTTTTCTTCCTACTAAACGAAGATTGAAGCTTGATCCTTCTGAGAAACT
CTATTTTCCTTGTGAGCTTTTTTCGAATCTCATTTTGATCGGAGTAGTTTCTCTGGAATT
ACAGTCTTTTTTTCATTGTCTAGGGTTTGTTTCTGAAGATTTGAGATCCATTGACACTAG
TCTAAAATTTCTATCTTTTAGTAGAATTTGAGTATTTGACTGATCTGATTATGTGGATTG
ATACATAAGTTTGATAATTGACTCAATAGGAAACATAAAGTTTGAAACTTTTTGGAAGTT
AAGAGCAGAATCTTGACATTGGATTATAATTCTAACTTACCATTACTCTAAATATGGTCT
TCCTT
   
Sequence Name 18-K027064-022-380-F11C-GS05
GenBank Accession LN494156 [GenBank]
Sequencing primer gene specific primer, GS05: 5'-TCAGAATGCTTAAAGGAAGACCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K027064-022-380-F11C-GS05
ATTTAGAGTAATGGTCAGTTAGAATTATAATCCAATGTCAAGATTCTGCTCTTAACTTCC
AAAAAGTTTCAAACTTTATGTTTCCTATTGAGTCAATTATCAAACTTATGTATCAATCCA
CATAATCAGATCAGTCAAATACTCAAATTCTACTAAAAGATAGAAATTTTAGACTAGTGT
CAATGGATCTCAAATCTTCAGAAACAAACCCTAGACAATGAAAAAAAGACTGTAATTCCA
GAGAAACTACTCCGATCAAAATGAGATTCGAAAAAAGCTCACAAGGAAAATAGAGTTTCT
CAGAAGGATCAAGCTTCAATCTTCGTTTAGTAGGAAGAAAAGACTTAGCAACAGATGAAA
CTGAAGGAGAAGGAGTCGGATGTTGATTTTGGCTTCATGTCCGGGAAATCTACATGGATC
AGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCA
ATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACG
ACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGA
AATCTTTATTTCGACGTGTCT

Last Updated on 06.12.2018 16:11