Sequences from confirmation amplicon


Confirmation sequences for line 380H01 and hit At2g38380 are shown.
Line ID 380H01
Locus At2g38380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16076997
Primers used for PCR 25vf - 8474, show primer details
   
Sequence Name 62-K117737-0022-380-H01C-25vf
GenBank Accession LN510843 [GenBank]
Sequencing primer gene specific primer, 25vf: 5'-ACAATTTCAAAGCTCAAACCAAAT-3'
Sequence (A. th genome BLAST matches underlined) 
>62-K117737-0022-380-H01C-25vf
AAAATACATAAAAAGTAAATTTTGTAGATCACTCTAACAAAATAATCTAAAACATTGAGT
TAACATTGTAATTTTATTACTTACAAATTACTATGTAAATTCTAGACTTAGTCTAGGAAT
GTGTCTGTCTAGCTTAGCTAGCTATCAATATATAAAAAGGAATTGCATTTTAAACACATG
CATATCTTAATTAGAGTTAGATGATGTAACATGAATTTTTTTACCGGAAAGAGCAACTAG
ATCGGAAGTGCGGTTTAGGCCAACGTCAGCAAAGGCTGTTTTAAGTTGAGTAAGATTGAA
AAACGGAGAGGGCAGAGCTGTATTAGCCAGAGCGAAGAAAGCTTCTACACTGTCTCTCCT
CCCCAACGGAACCGGCCACCATGGACCTCCCGACTATATAAAACATAGACTTTTAATTGA
TAACATTTTCGATTAGTTTAATAAAAGATACGTCATGTATGTATATTTATATACCAAAAG
CACTGATATTTGAGAGGCGATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATTAT
   
Sequence Name 30-K117736-0022-380-H01C-8409
GenBank Accession LN499896 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K117736-0022-380-H01C-8409
GCTTTTGGTATTAATATACATACATGACGTATCTTTTATTAAACTAATCGAAAATGTTAT
CAATTAAAAGTCTATGTTTTATATAGTCGGGAGGTCCATGGTGGCCGGTTCCGTTGGGGA
GGAGAGACAGTGTAGAAGCTTTCTTCGCTCTGGCTAATACAGCTCTGCCCTCTCCGTTTT
TCAATCTTACTCAACTTAAAACAGCCTTTGCTGACGTTGGCCTAAACCGCACTTCCGATC
TAGTTGCTCTTTCCGGTAAAAAAATTCATGTTACATCATCTAACTCTAATTAAGATATGC
ATGTGTTTAAAATGCAATTCCTTTTTATATATTGATAGCTAGCTAAGCTAGACAGACACA
TTCCTAGACTAAGTCTAGAATTTACATAGTAATTTGTAAGTAATAAAATTACAATGTTAA
CTCAATGTTTTAGATTATTTTGTTAGAGTGATCTACAAAATTTACTTTTTATGTATTTTT
TTTCTTTCTTATAGATATGATTTGGTTTGAGCTTGGAAATTGT

Last Updated on 06.12.2018 16:11