Sequences from confirmation amplicon


Confirmation sequences for line 381B01 and hit At3g05550 are shown.
Line ID 381B01
Locus At3g05550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:1613285
Primers used for PCR 13xu - 8474, show primer details
   
Sequence Name 82-K119579-0022-381-B01C-13xu
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 13xu: 5'-CTATATCAGAAACTGAAGCCCGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K119579-0022-381-B01C-13xu
TTGAACAACCCTAGAAGGTGAAATTCTCTGAAGAGACGAGCGGCGTCTTTTTTGCTTTGG
GGAGAATGAAACAGATGGCTAGGGTTTTTGCCCATTTAAAGTGGTCTTGTTGCCCTAATT
TATATTTCTATAAATAACCACAAGATCCCCAAACTTTTGTTAAATCCAAAAATAATCCCA
ATTGTTTTTAAATTGTAATAACTCCCACAACTCGTTAGACAAGTGAGGATATTTATTCTT
TTGTGTTCCACCAACAAATGCTAGTTGTTCAATTTAATCATGGATTTGTTTTCAGTACAT
TATCTCTTAGAAGTCTGTATATTGTGTGCAATCTACAACACATGACAATCCATCTGAATG
TATCAAAAGAATGATATACTGTAATAAGCAAAAATTTGTTTTTGTCAATTACATGAAAAT
TTCCCTGAAAGGTCTACAAAAATTCTCTCTAGCTACGAGACCTTGTACCAAACAGAAAAC
GCTGCAACCAACCATTGCAGCTTAGGAGTTTAGAGTTTTTAGTCTTTGTGAGATAAATTT
TCCGGTGGCAGAAACTTCGGATAGCGGTTGGTAGCTTCAGTTTTGTGGTCATAGTACTCC
ACCACGGCTGCTCCAGCCAAAGCAGCTAAAGTCAGCGCCTGAGCGTGCAACCTGAGAAGA
AGCTCGGTGAGAAAAGTGCTTCGTACAGTACCATGTGACTGGATGTCTCGAACAAATGAT
TTGGCTAAGAACATAAAATTTATGTCGATAACAACTATAGCTTACAGGCAAGAACAGAGA
AATGTACATGCTTTTGGGACATGGAGATGATGATGTTGCTCAACTATAAATGTTCATGGC
TACAGAAAATGTTTCAAAATGCAAATGAGACTCAAAAACTTTACAAAAAAATCATTCAAC
CATTTATAATCAATCAATCCCTTTTGATTAGAAGATTCACCTGGCGTGGATGATCTTGAC
ACTTGTTTTCATGGCAGGTTGGG
   
Sequence Name 58-K119578-0022-381-B01C-8409
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K119578-0022-381-B01C-8409
CATTGTTTTTTGATTGAAATAAGAGCCACT

Last Updated on 06.12.2018 16:11