Sequences from confirmation amplicon


Confirmation sequences for line 381B12 and hit At4g10457 are shown.
Line ID 381B12
Locus At4g10457 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6467123
Primers used for PCR 31qt - 8474, show primer details
   
Sequence Name 28-K115781-0022-381-B12C-31qt
GenBank Accession LN498987 [GenBank]
Sequencing primer gene specific primer, 31qt: 5'-TAAATGGACTTAGAGGTTTTAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K115781-0022-381-B12C-31qt
GCTCCTCTACAGCTGTCTCCAGCTGCCTCCAATTTGTCACCTTCCACCAATTTGGATTGG
GCTTAACCCGCGTGCAACTTTACCAAACCAAGACAAGAATGCTTTAGTCGTGCGACTCAT
AACCCTGGTTGGCGCTAACGGCTCCTTCTTTGTCTTGTGGTCCTGCTGTTGACGTGTCCC
CTTTGCTGAGTTGGCATTTCTTCTTCCTCAAGCTTCTTCATGTTGTGTTCTTTGTTGCTA
AATCTTAACAAATAGCCACATATATTTTTATCTTGTTGAGTATATTAATTATGAAGATTG
TTGGCAAAGAAAACTTAATATACTTTCTATGAGAGTAAAACAAAACACAAATTTTTGAAG
CTCAAAGAGACTATTTGTTTAGACTATAAGTCATGAAATATGGAGTTTTGTTTATGGTTT
CTTGTGGTGTCATGTTTCTAATTCTAAGCCATGTTGAAGGTAACTTGTTTCCTTATTTTG
TGTTATCAACTGTTTTGTATATATATTTTCATAGTCTTAACATTTTATTTTTGTGGTTGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATT
   
Sequence Name 04-K115780-0022-381-B12C-8409
GenBank Accession LN488053 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K115780-0022-381-B12C-8409
TCAACCACAAAAATAAAATGTTAAGACTATGAAAATATATATACAAAACAGTTGATAACA
CAAAATAAGGAAACAAGTTACCTTCAACATGGCTTAGAATTAGAAACATGACACCACAAG
AAACCATAAACAAAACTCCATATTTCATGACTTATAGTCTAAACAAATAGTCTCTTTGAG
CTTCAAAAATTTGTGTTTTGTTTTACTCTCATAGAAAGTATATTAAGTTTTCTTTGCCAA
CAATCTTCATAATTAATATACTCAACAAGATAAAAATATATGTGGCTATTTGTTAAGATT
TAGCAACAAAGAACACAACATGAAGAAGCTTGAGGAAGAAGAAATGCCAACTCAGCAAAG
GGGACACGTCAACAGCAGGACCACAAGACAAAGAAGGAGCCGTTAGCGCCAACCAGGGTT
ATGAGTCGCACGACTAAAGCATTCTTGTCTTGGTTTGGTAAAGTTGCACGCGGGTTAAGC
CCAATCCAAATTGGTGGAAGGTGACAAATTTGGAGGCAGCTTGGAGACAGCTGTAGAGGA
GCTTGTAGAGGCAAACCAAGAAGGATCCAGAAGGGAACCTAGGTTAAAACCTCTAGTCTC
ATT

Last Updated on 06.12.2018 16:11