Sequences from confirmation amplicon


Confirmation sequences for line 381C02 and hit At3g52950 are shown.
Line ID 381C02
Locus At3g52950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:19636051
Primers used for PCR BW80 - 8474, show primer details
   
Sequence Name 54-K106290-0022-381-C02C-8409
GenBank Accession LN508487 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K106290-0022-381-C02C-8409
GCTCTACGCAAGAGCTGAATCCCAAAATTTCTGCATCATCGTGTTTGCCATATCATTTAC
AGCTCCAGAACTGTTCTCCACCATTGAAATAGCTGCGTGAGTAATCTGAAGAACGTCTAC
ACAAGCTGCAGCTGATCCATCTTTATCTATAATTGGAAGATGTAGAAACTTTCCATCATG
CATTGTATGCAACGCATCCAGAATCGTTGTCTCTAGCGATGCACATTCAGGATTCGGTGT
CATTACCTTCTCGACAAGAGTCAATTCAGGAGATAAATTTTGTGCCACCACTCGCATCAG
AATGTCCTTTGAAGTCAAGATTCCACTGATTTTGTTCCCCGTGGAAATGATTACAGAGTT
AACCCGCAAATCCCTCATCCTTTTCGCAGCAACTGAAACAGGATCTGATGGTGCTACAAG
TGCAACCTTCGAATTGTCTGTAATAATCGTTGACAAGGCGGGTTTAAACATTCTCTCCCT
CAAGGTTTCAATGAAAGCATACGGTGCAGAATATCCGCTTCCCCATTGCTTCTCCACACC
TTCCACTGCAGCAGCTAAAGCACTACCTTGCTCTGCAGCTTTCT
   
Sequence Name 54-K106291-0022-381-C02C-BW80
GenBank Accession LN508488 [GenBank]
Sequencing primer gene specific primer, BW80: 5'-TATTTCTAGGATGGAGAAAGCTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>54-K106291-0022-381-C02C-BW80
AGGTGTGGAGAACAATGGGGAAGCGGATATTCTGCACCGTATGCTTTCATTGAAACCTTG
AGGGAGAGAATGTTTAAACCCGCCTTGTCAACGATTATTACAGACAATTCGAAGGTTGCA
CTTGTAGCACCATCAGATCCTGTTTCAGTTGCTGCGAAAAGGATGAGGGATTTGCGGGTT
AACTCTGTAATCATTTCCACGGGGAACAAAATCAGTGGAATCTTGACTTCAAAGGACATT
CTGATGCGAGTGGTGGCACAAAATTTATCTCCTGAATTGACTCTTGTCGAGAAGGTAATG
ACACCGAATCCTGAATGTGCATCGCTAGAGACAACGATTCTGGATGCGTTGCATACAATG
CATGATGGAAAGTTTCTACATCTTCCAATTATAGATAAAGATGGATCAGCTGCAGCTTGT
GTAGACGTTCTTCAGATTACTCACGCAGCTATTTCAATGGTGGAGAACAGTTCTGGAGCT
GTAAATGATATGGCAAACACGATGATGCAGAAATTTTGGGATTCAGCTCTTGCGTTAGAG
CCACCTGATGATTCTGACACTCAAAGTGAAATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
Primers used for PCR 07bg - 3144, show primer details
   
Sequence Name 41-K107114-0022-381-C02C-3144
GenBank Accession LN504095 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K107114-0022-381-C02C-3144
ACTATCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGG
TACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGATTGTCGTTTCC
CGGTCACCTGATGATGCATCATTCAGATATAGGGAAGCTCAGTTCTTACCCATCTCTAGG
ACTAGGGAATTCGTTTTCGTTCAAATTTGAAGACCTTAAGGGCCGTGTACATCGGTTTAC
TTCTGGAGCTGAAAACCTCGAAGAGCTGATGGGTATTGTGATGCAAAGAATTGGCAGCGA
CAATAACAATGTGGAACAACGGCCTCAGATTATATATGAGGACGATGAAGGTGATAAGGT
TTTGATTACATCGGATAGCGATTTGGTTGGTGCTGTTACTCTTGCTAGGTCTACAGGACA
AAAGGTTTTGAGGCTGCATCTGGACTTCACTGAGTCAACAAGGAGCTTGAGCTCAGAAAC
CACCCAACTCAAGAAAGGTGATTCTCGAGACAGAGGAAGCGGATGGGTTTCATGGCGTGG
TGGTGTGGTGGTTACAGGA
   
Sequence Name 57-K107115-0022-381-C02C-07bg
GenBank Accession LN509433 [GenBank]
Sequencing primer gene specific primer, 07bg: 5'-GTTAGGACAACAGCTCCTGTAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K107115-0022-381-C02C-07bg
GCTTCTCTGTCTCGAGAATCACCTTTCTTGAGTTGGGTGGTTTCTGAGCTCAAGCTCCTT
GTTGACTCAGTGAAGTCCAGATGCAGCCTCAAAACCTTTTGTCCTGTAGACCTAGCAAGA
GTAACAGCACCAACCAAATCGCTATCCGATGTAATCAAAACCTTATCACCTTCATCGTCC
TCATATATAATCTGAGGCCGTTGTTCCACATTGTTATTGTCGCTGCCAATTCTTTGCATC
ACAATACCCATCAGCTCTTCGAGGTTTTCAGCTCCAGAAGTAAACCGATGTACACGGCCC
TTAAGGTCTTCAAATTTGAACGAAAACGAATTCCCTAGTCCTAGAGATGGGTAAGAACTG
AGCTTCCCTATATCTGAATGATGCATCATCAGGTGACCGGGAAACGACAATCTGATAGTT
TAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAA
TGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCT
TACGTCAGTGGAGAT

Last Updated on 06.12.2018 16:11