Sequences from confirmation amplicon


Confirmation sequences for line 381G10 and hit At2g23300 are shown.
Line ID 381G10
Locus At2g23300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9916364
Primers used for PCR EQ41 - 8474, show primer details
   
Sequence Name 30-K103006-0022-381-G10C-8409
GenBank Accession LN499671 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K103006-0022-381-G10C-8409
TTACATTGAATATGCTAACATGAACACAGTAGCAAAACCCTGGTGATGCTAGCGTGCGAC
TTGATCTTATGCTAACATGTGCCTTTATCATAAAGCGAGGAAAAAGATTGAAATGTGAAA
GAGATGATTTTGACTCACTGTAACGGGCGTTGACGAGACTGCCGTTAGGAACAAAATCGT
AAATGACCAATTTCTCGTCGGTGCCCCAATAGAATCCACGAAGACGTACCAAATTCGGGT
GAACCAATTTACCAATAGCTCGAATATGTGCCTCAAAGTCTTTAAACCGGCGTTGTTGAC
TCAAACCATTCTCACCTAACCGACGAACCGCGAGAACCGTACCGTCCTCAAGAACAGTCT
TGTACATTATACTCGAACCAGTGGCTCCTAAAATGTAAGCAGAAGCCTTAAGCAAAGTTT
CACTNTCAAGCT
   
Sequence Name 78-K103007-0022-381-G10C-EQ41
GenBank Accession LN513672 [GenBank]
Sequencing primer gene specific primer, EQ41: 5'-GAGCTTGAAGTTGAAACTTTGCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>78-K103007-0022-381-G10C-EQ41
AGTATAATGTACAGACTGTTCTTGAGGACGGTACGGTTCTCGCGGTTCGTCGGTTAGGTG
AGAATGGTTTGAGTCAACAACGCCGGTTTAAAGACTTTGAGGCACATATTCGAGCTATTG
GTAAATTGGTTCACCCGAATTTGGTACGTCTTCGTGGATTCTATTGGGGCACCGACGAGA
AATTGGTCATTTACGATTTTGTTCCTAACGGCAGTCTCGTCAACGCCCGTTACAGTGAGT
CAAAATCATCTCTTTCACATTTCAATCTTTTTCCTCGCTTTATGATAAAGGCACATGTTA
GCATAAGATCAAGTCGCACGCTAGCATCACCAGGGTTTTGCTACTGTGTTCATGTTAGCA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTACATTC

Last Updated on 06.12.2018 16:11