Sequences from confirmation amplicon


Confirmation sequences for line 381H10 and hit At4g25910 are shown.
Line ID 381H10
Locus At4g25910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:13164442
Primers used for PCR CC04 - 8474, show primer details
   
Sequence Name 57-K022913-022-381-H10C-8409
GenBank Accession LN509286 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K022913-022-381-H10C-8409
TAGATTTCCCGGACATGAAGCCATTTACAATTGAACTCATTGCAAACCAGACCAATCTCT
TTAGAACTTTAGTTGTGATTTAGATTCTTGTTATGTTTGGTTTAGTATTTGGTCACGTGA
GCTGCGTCATGCCGTTAACGGAAGAGAATGTGGAGAGAGTGTTAGACGAAGTACGACCAT
CTCTAATGGCCGACGGAGGAAACGTGGCGTTGCACGAAATCGACGGACTTGTGGTGGTTT
TAAAGCTACAAGGAGCTTGTGGTTCGTGTCCTAGCTCATCAATGACGTTGAAGATGGGAA
TCGAGAGTCGTCTTCGAGACAAGATTCCAGAGATCATGTCCGTTGAGCAGTTTCTTGAAT
CCGAGACAGGAGGTTTAGAGCTGAACGATGAGAACATTGAGAAGGTTTGGTTTTTTAAAG
GTTTAGTTATTTTTGGTTTATTAGCCCCTTGGTTTTGAAATTGA
   
Sequence Name 61-K022913-022-381-H10C-CC04
GenBank Accession LN510406 [GenBank]
Sequencing primer gene specific primer, CC04: 5'-ACCATTCAGATCAATTTCAAAACC-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K022913-022-381-H10C-CC04
GGGCTAATAAACCAAAAATANACTAAACCTTTAAAAAACCAAACCTTCTCAATGTTCTCA
TCGTTCAGCTCTAAACCTCCTGTCTCGGATTCAAGAAACTGCTCAACGGACATGATCTCT
GGAATCTTGTCTCGAAGACGACTCTCGATTCCCATCTTCAACGTCATTGATGAGCTAGGA
CACGAACCACAAGCTCCTTGTAGCTTTAAAACCACCACAAGTCCGTCGATTTCGTGCAAC
GCCACGTTTCCTCCGTCGGCCATTAGAGATGGTCGTACTTCGTCTAACACTCTCTCCACA
TTCTCTTCCGTTAACGGCATGACGCAGCTCACGTGACCAAATACTAAACCAAACATAACA
AGAATCTAAATCACAACTAAAGTTCTAAAGAGATTGGTCTGGTTTGCAATGAGTTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAG
CGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTA
TAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACA
T

Last Updated on 06.12.2018 16:11