Genotyping and primer details


Genotyping and primer details for 381H10 - At4g25910
Line ID 381H10
Locus At4g25910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13164442 of line 381H10 Chr4:13164442
   
Insertion allele amplicon 8474 - CC04
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CC04: 5'-ACCATTCAGATCAATTTCAAAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13164876
   
wt amplicon (over InsPos) 20fo - CC04
wt amplicon start Chr4:13163964
wt amplicon end Chr4:13164876
wt amplicon size 913 bp
Locus specific primer CC04: 5'-ACCATTCAGATCAATTTCAAAACC-3'
2nd locus specific primer 20fo: 5'-TCCACCAATGAAAAGTTCTCTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11