Sequences from confirmation amplicon


Confirmation sequences for line 382C01 and hit At2g25350 are shown.
Line ID 382C01
Locus At2g25350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10798415
Primers used for PCR AC83 - 8474, show primer details
   
Sequence Name 77-K104358-0022-382-C01C-AC83
GenBank Accession LN513545 [GenBank]
Sequencing primer gene specific primer, AC83: 5'-TCGTGAACTATTCCAAGGAACTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K104358-0022-382-C01C-AC83
GTTTTACGGGTTCAGGTAAGTGTGCAATCTCCAGAAGGTGTTAGCACAATGCGAGGAATT
CTGAGGAGATTTAATGATTTTGTAAAGCTTTTAGCTGATGTAAGATTCTCTCAATCTTTC
TCAACTCAATGATATTCAACATGATTTGTAGTTGTTTTTGGTCAGTGTGATTCAAACAAG
TTAACTGCAATGTGTTTTATGGCATATGATTTACAGCTCAAAAGAGCATTTCCTAGGAAA
AGTTTTCCATCAGCTCCTCCTAAAGGATTTTTGCGTGTCAAAAGTAGAGATATGTTAGAA
GAGGTATGATGGTCAACAATGAAGAGCTACATGGATATATTCAATTGTAAATGGCTTCAT
GTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAG
AGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 85-K104359-0022-382-C01C-8409
GenBank Accession LN514542 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K104359-0022-382-C01C-8409
TTACATTGAATATATCCATGTAGCTCTTCATTGTTGACCATCATACCTCTTCAACATATC
TCTACTTTTGACACGCAAAAATCCTTTAGGAGGAGCTGATGGAAAACTTTTCCTAGGAAA
TGCTCTTTTGAGCTGTAAATCATATGCCATAAAACACATTGCAGTTAACTTGTTTGAATC
ACACTGACCAAAAACAACTACAAATCATGTTGAATATCATTGAGTTGAGAAAGATTGAGA
GAATCTTACATCAGCTAAAAGCTTTACAAAATCATTAAATCTCCTCAGAATTCCTCGCAT
TGTGCTAACACCTTCTGGAGATTGCACACTTACCTGAACCCTGTAAAACTGTACCTCAAG
AATGTCACATATATCGAGCAAGAGTTCCTTGGAATAGTTCAC

Last Updated on 06.12.2018 16:11